低氧训练对大鼠骨骼肌血红素氧合酶mRNA表达的影响

目的:探讨不同低氧训练模式对机体骨骼肌血红素合酶(HO-1)mRNA表达的影响。方法:选用6周龄SD雄性大鼠120只,经3周适应性训练和力竭实验筛选出90只,随机分成9组:常氧安静对照组、持续低氧安静组、间歇低氧安静组、低住低练组、高住高练组、高住低练组、低住高练组、高住高练后复氧训练组、高住低练后复氧训练组。采用常压低氧舱以13.6%的氧浓度(相当于海拔3500m的氧浓度)进行低氧训练,根据血乳酸-速度曲线确定大鼠常氧训练的强度为35m/min,低氧训练的强度为30m/min。低氧训练持续时间为6周,每周训练5天。第6周末最后一次运动后休息48h后处死、取材。采用实时荧光定量PCR技术测试大鼠骨骼肌HO-1mRNA表达。结果:与常氧安静对照组相比,低住低练组大鼠骨骼肌HO-1mRNA表达显著升高(P<0.05),高住高练组、低住高练组非常显著升高(P<0.01);高住低练组与低住低练组比较显著降低(P<0.05);高住高练后复氧训练组大鼠骨骼肌HO-1mRNA表达与高住高练组相比显著降低(P<0.01),基本回到常氧安静对照组水平。结论:高住高练和低住高练可提骨骼肌HO-1mRNA表达。

Effect of Hypoxic Training on the Expression of HO-1 in Skeletal Muscle of Rats

Objective The purpose of this study was to observe the effects of different hypoxia training modes on the HO-1(heme oxygenase 1)mRNA in skeleton muscle,in order to provide theoretical and applying basis for the hypoxic training.Methods Ninety from 120 male SD rats(6 weeks old)were selected through adaptable training and exhaustive exercise testing,and randomly divided into 9 groups:normoxic untrained group,persistent hypoxic untrained group,intermittent hypoxic untrained group,normoxic training group,living at high altitude and training at high altitude group(HiHi),living at high altitude and training at low altitude group(HiLo),living at low altitude and training at high altitude group(LoHi),normoxic training after living at high altitude and training at high altitude group,and normoxic training after living at high altitude and training at low altitude group.Hypoxic chamber contained 13.6% of oxygen,simulating of altitude of 3500m.Training rats were forced to run on the treadmill 5d/week.The speed of the treadmill was 30m/min under hypoxia and 35m/min under normoxia.The rats were sacrificed after 6 weeks.RQ-PCR was adopted for testing the mRNA of HO-1 in skeletal muscle.Results Comparing with the normoxic untrained group,the mRNA level of HO-1 increased significantly in normoxic training group(P <0.05),especially in HiHi group(P <0.01)and LoHi group(P <0.01),and decreased significantly in HiLo group(P <0.05).Comparing with the HiHi group,the mRNA level of HO-1 decreased significantly in normoxic training group after living at high altitude and training at high altitude group(P<0.01).Conclusion Living at high altitude and training at high altitude,and living at low altitude and training at high altitude increase level of HO-1 in skeletal muscle.