The effect of the surface morphology of Ti-implants on the proliferation activity of fibroblasts and osteoblasts

It is known that surface morphology greatly influences the osseointegration of dental implants. The goal of the experiments conducted by the authors was to study in vitro the effect of various surface modifications on the activity of bone and connective tissue cells. In the experiments they introduced NIH3T3 fibroblast and MCH3T3 osteoblast cells were cultured the surface of ten titanium disks with various morphology in 24-multiwell plates. The cells were let grow on the surface of the disks for two days in a culture medium. One group of the disks underwent scanning electronmicroscopy and the changes in the number and form of cells on the surfaces were studied under various magnifications. From the surface of the second group the cells were lysed and were counted in a Bürker's chamber. After counting the cells they were lysed in a lysis buffer and their protein concentrations were measured with the help of a spectrophotometer. The authors found that only a morphological study of the cells is possible under SEM, they could not detect a quantitative difference in the number or activity of the cells. Upon counting the cells and determining their protein concentration the best result were yielded by the surface roughened by aluminium oxide. In growing fibroblasts the titanium oxide treated surfaces provided good results, while in the case of the osteoblasts the laser treated disks were more successful.