TC指数在改良LONGA线栓法局灶性脑缺血/再灌注动物模型制备中的应用

目的观察TC指数定位血管线栓法对大鼠局灶性脑缺血模型制备的影响。方法300只雄性SD大鼠随机分为预试验组30只、原始方法组120只(按LONGA氏血管线栓法)、改良方法组150只(按动物门齿根部T点距颈总动脉分叉点C点长短而定,简称TC指数定位法)；比较两种不同造模法对血管线栓局灶性脑缺血/再灌注实验大鼠的成功率及形态学的变化。结果TC指数定位法改良的LONGA氏血管线栓再灌注制模法,比原始的LONGA氏血管线栓法大鼠脑缺血/再灌注模型成功率高,直线相关分析及统计学上均具有显著性意义；形态学表现差异无显著性。结论TC指数定位法的LONGA氏血管线栓法大鼠脑缺血/再灌注模型是一种较好的制模方法,值得实验研究中推广应用。

Application of TC Index Location on LONGA's Animal Model of Regional Experimental Cerebral Ischemia and Reperfusion

Objective To study the TC index location on a reform LONGA's animal model of regional exper- imental cerebral ischemia and reperfusion.Methods A total of 300 SD male rats were randomly divided into the pre-test group (n=30),the original method group (n=120,Longa's method) and the reform method group n= 150,TC index location is defined by the length of rat incisor teeth root (T point) to the common carotid artery bi- furcation (C point),the blood flow of a unilateral middle cerebral artery in the rats was reversibly blocked by the TC index location].The morphologic changes and the successful rate of the two methods to establish the rat model were compared.Results The successful rate of TC index location modified model method was superior to that of o- riginal LONGA's method,with significant difference in linear correlation analysis.Conclusion The reform LON- GA's animal model of regional experimental cerebral ischemia and reperfusion by the TC index location is a good method,and is worth applying in experimental study.