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Improved transformation of C3H10T1/2CL8 cells by direct- and indirect-acting carcinogens |
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| Authors: | Nesnow, Stephen Garland, Helen Curtis, Gaynelle |
| Affiliation: | 1Carcinogenesis and Metabolism Branch, Genetic Toxicology Division, Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park NC 27711 2Northrop Services, Inc. Environmental Sciences, Box 12313, Research Triangle Park NC 27709, USA. |
| Abstract: | Oncogenic transformation of C3H10T1/2CL8 cells was improvedby treating the cells 5 days after seeding. Benzo[a]pyrene-inducedtransformation was increased 3.5-fold by this method, comparedwith treating the cells 1 day after seeding. N-Methyl-N' -nitro-N-nitrosoguanidine,which does not transform asynchronous cultures of C3H10T1/2CL8cells when administered 1 day after seeding, produced an averageof 1 focus/dish, with 61% of the dishes exhibiting foci, whenadministered 5 days after seeding. Propane sultone and aflatoxinB1 also produced marked transformation responses when administered5 days after seeding. However, 4-dimethylaminoazobenzene didnot induce transformation when administered either 1 day or5 days after seeding. With all chemicals examined, clonal cytotoxicitywas reduced when they were administered 5 days after seeding.These results indicate the utility of this new procedure forthe qualitative analysis of the transforming ability of chemicals. |
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