| 下调CD147通过Bcl-2途径诱导宫颈癌细胞SiHa细胞凋亡的研究 |
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| 引用本文： | 周希,吕洁玉,龚宝兰. 下调CD147通过Bcl-2途径诱导宫颈癌细胞SiHa细胞凋亡的研究[J]. 南方医科大学学报, 2010, 30(7): 1695 |
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| 作者姓名： | 周希 吕洁玉 龚宝兰 |
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| 作者单位： | 郧阳医学院附属人民医院妇产科,湖北,十堰,442000;郧阳医学院附属人民医院妇产科,湖北,十堰,442000;郧阳医学院附属人民医院妇产科,湖北,十堰,442000 |
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| 摘 要： | 目的 研究下调CD147表达后宫颈癌SiHa细胞凋亡变化.方法 设计、合成两对CD147编码基因的反向重复序列,运用瞬间转染方法抑制SiHa中CD147表达,运用RT-PCR、Western blotting方法检测干扰后CD147、Bcl-2、Bim及caspase-3表达改变,流式细胞术检测干扰后肿瘤细胞的凋亡情况.结果 SiRNA sequence 1、2均有效抑制CD147基因表达(P<0.05),伴随着Bcl-2 mRNA和蛋白表达水平降低(P<0.05),干扰后caspase-3 mRNA、Bim水平升高(P<0.05),活性caspase-3蛋白及Bim水平增高(P<0.05),肿瘤细胞凋亡增加,以细胞早期凋亡改变最为明显(P<0.05).结论 沉默CD147基因表达可部分通过Bcl-2途径诱导SiHa细胞凋亡.
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| 关 键 词： | CD147 Bcl-2 Bim Caspase-3 SiRNA |
Down-regutation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway |
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| ZHOU Xi,LV Jie-yu,GONG Bao-lan. Down-regutation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway[J]. Journal of Southern Medical University, 2010, 30(7): 1695 |
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| Authors: | ZHOU Xi LV Jie-yu GONG Bao-lan |
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| Affiliation: | ZHOU Xi,LV Jie-yu,GONG Bao-lan Department of Obstetrics and Gynecology,People's Hospital Affiliated to Yunyang Medical College,Shiyan 442000,China |
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| Abstract: | Objective To investigate the changes in SiHa cell apoptosis after inhibition of CD147 expression. Methods RNA interference (RNAi) technique was used to down-regulate CD147 expression in SiHa cells, and RT-PCR and Western blotting were used to detect expression of CD147, Bcl-2, Bim and caspase-3; the percentage of cell apoptosis were detected by flow cytometry. Results SiRNA sequence 1, 2 inhibited CD147 expression in SiHa cells effectively (P<0.05), resulting also in down-regulated expression of Bcl-2 (P<0.... |
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| Keywords: | CD147 Bcl-2 Bim Caspase-3 SiRNA |
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