烟焦油对体外人视网膜色素上皮细胞的损害

目的:研究烟焦油对体外培养的人视网膜色素上皮细胞(human retinal pigmentepithelium,HRPE)超微形态结构的影响,以及趋化因子MCP-1的细胞外表达水平。方法:将体外培养的HRPE分为空白对照组、二甲亚砜(dime-thylsulfoxide,DMSO)对照组和焦油实验组,其中焦油实验组中焦油浓度分别为1,10,50mg/L,经48h培养后,与空白对照组及DMSO对照组进行比较,使用倒置显微镜及透射型电子显微镜观察HRPE的形态以及超微结构变化,并用ELISA法选择检测各组培养上清中细胞因子MCP-1的表达量。结果:倒置显微镜显示焦油实验组随着焦油浓度的增大,发生变性坏死的HRPE细胞数量逐渐增多,并且细胞的形态发生明显改变;电镜显示焦油实验组随着焦油浓度的增大,RPE细胞形态扁平,微绒毛减少,胞质稀疏、减少,细胞核细长、核染色质稀疏,染色质浓缩,可见空泡,细胞呈萎缩状态;ELISA结果显示随着焦油浓度的增大,RPE分泌至胞外的MCP-1逐渐减少,差异有统计学意义(P<0.05)。结论:烟焦油可改变视网膜色素上皮细胞正常的形态和生理功能,造成色素上皮细胞的严重损害。

Damage of cigarette tar on human retinal pigment epithelium in vitro

AIM:To investigate the effect of cigarette tar on the ultrastructure and the expression of monocyte chemotactic protein-1(MCP-1)in human retinal pigment epithelium(HRPE)in vitro.·METHODS:The cultured HRPE were divided into blank group,DMSO group and cigarette tar experimental group.The concentrations of tar in cigarette tar experimental group were 1,10,50mg/L.After HRPE were treated with cigarette tar for 48h,the cellular morphology of HRPE was observed by inverted microscope and their ultrastructure was analyzed by transmission electron microscope,while ELISA kit is used to determine the levels of MCP-1 in the supernatants in the three groups.·RESULTS:The quantity of HRPE,which degenerated or necrosed in cigarette tar experimental group,increased with the rising of tar’s concentration in inverted microscope.The transmission electron microscope showed that HRPE became flat in morphous with reduced microvilli,their cytoplasm were rarities and diminished while their cellular nucleus became slender,with rarities and psychotics chromatin.And the expression of MCP-1 in the supernatants was reduced significantly with the rising of tar’s concentration(P<0.05).·CONCLUSION:Cigarette tar can change HRPE’s normal morphous and physiological function,and it can cause HRPE serious damage.Smoking may damage retinal by affecting retinal pigment epithelium.