| 高效液相色谱法测定复方苦参洗剂中苦参碱含量 |
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| 引用本文： | 左力. 高效液相色谱法测定复方苦参洗剂中苦参碱含量[J]. 辽宁中医学院学报, 2013, 0(8): 49-50 |
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| 作者姓名： | 左力 |
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| 作者单位： | 辽宁中医药大学附属医院,辽宁沈阳110032 |
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| 摘 要： | 目的：建立复方苦参洗剂中苦参碱的含量测定方法。方法：反相高效液相色谱法,采用Diamonsil不锈钢C18色谱柱（4.6 mm×150 mm,5μm）,以甲醇-水-三乙胺（45∶55∶0.05）为流动相,紫外-可见检测器测定波长为220 nm,流速为0.6 mL/min。结果：苦参碱在12.60~126.00 mg.L-1与峰面积之间线性关系良好,r=0.9995,回收率为99.65%,RSD为2.3%。结论：本方法用于复方苦参洗剂中苦参碱含量的测定,简单、快速、准确。
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| 关 键 词： | 高效液相色谱法 复方苦参洗剂 苦参碱 |
HPLC Determination of Matrine in Compound Matrine Lotion |
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| Affiliation: | ZOU Li ( Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China ) |
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| Abstract: | Objective ： To establish a method for the determination of matrine compound matrine lotion. Methods： RP-HPLC using the Diamonsil stainless C18 column （4.6 mm×150 mm,5 μ m ）, methanol - water - of triethylamine （ 45 ： 55 ： 0.05 ） mobile phase, UV - visible detector.The measurement wavelength was 220 nm, and the flow rate was 0.6 mL/min. Results： Matrine between 12.60-126.00 mg·L-1 and the peak area had a good linear relationship （r=0.9995）, the recovery rate was 99.65%, RSD was 2.3%. Conclusion ： The method for determination of matrine in compound sophora lotion is simple, fast and accurate. |
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| Keywords: | high performance liquid chromatography compound sophora lotion matrine |
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