姜黄素长循环脂质体的制备及评价

目的:研制姜黄素长循环脂质体。方法:采用乙醇注入法制备姜黄素长循环脂质体,应用微柱离心法测定包封率,以包封率为指标,分别考察缓冲液种类、药脂比、胆固醇用量、不同相对分子质量的PEG-胆固醇、不同pH、离子强度等对脂质体的影响,在此基础上用正交设计[L₉(4³)]对处方进行优化。结果:经乙醇注入法制得的脂质体的包封率为(88.27±2.16)%,平均粒径为(136±18)nm,粒度分布均匀,呈单峰分布,4℃放置30 d包封率无明显变化。结论:姜黄素长循环脂质体制备工艺简单可行,姜黄素长循环脂质体制剂学性质稳定。

Preparation and study in vitro of long-circulating nanoliposomes of curcumin

Objective:To prepare the long-circulating nanoliposomes of curcumin.Method:The long-circulating nanoliposomes were prepared by ethanol infusion and the encapsulation efficiency was determindated by the mini-column centrifugation.The effect of some factors on the encapsulation efficiency,such as the buffer solutions,the weight ratio of curcumin to SPC,the weight ratio of SPC to Chol,the pH of buffer solution and the iron strength of water phase,was investigated respectively.Then the formulation was optimized by orthogonal design.Result:The encapsulation efficiency of the curcumin liposomes was(88.27±2.16)%,and the average diameter of the liposomes was(136±18) nm.There was no change on encapsulation efficency within 30 d.Conclusion:The preparation of curcumin liposomes was easy and practicable and the pharmaceutical characterization showed that the curcumin liposomes are stable.