Purification and characterization of an agglutinin of the soft coral Sinularia species. |
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| Authors: | R Goto K Muramoto M Yamazaki H Kamiya |
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| Affiliation: | School of Fisheries Sciences, Kitasato University, Iwate, Japan. |
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| Abstract: | ![]()
A D-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca(++)-independent. Sinularian promoted binding of macrophages to tumor cells. |
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