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The use of human antigen-specific monoclonal antibodies in an enzyme-linked immunosorbent assay for the determination of anti-HBsAg antibody subclasses |
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| Authors: | Mats A. A. Persson Lennart Hammarstr m C. I. Edvard Smith Elisabeth A. M. Stricker |
| Affiliation: | 1 Department of Clinical Immunology, Karolinska Institute at Huddinge Hospital, Huddinge, Sweden 2 Department of Immunology, Stockholm University, Stockholm, Sweden 3 Department of Immunobiology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands |
| Abstract: | A human monoclonal anti-HBsAg antibody (IgGl, λ) was used as a reference for an ELISA determination of the serum levels of anti-HBsAg antibodies in human volunteers after vaccination with H-B vax. The IgG subclass distribution of specific antibodies showed a marked dominance of IgGl antibodies. In addition, small amounts of specific IgG4 antibodies were occasionally found, suggesting a different pattern from that found after natural disease. The novel use of a human monoclonal antibody to measure specific antibodies may give a more accurate determination of specific IgG subclass levels than previously available methods. |
| Keywords: | IgG subclass Anti-HBsAg antibody ELISA Monoclonal antibody, human |
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