利用核酸恒温扩增技术检测下呼吸道感染病原菌的分布特征

目的 探讨环介导温扩增技术(Loop-mediate amplification,LAMP)检测呼吸道病原菌在临床中实际应用价值.方法 收集2018年3月-2019年4月徐州医科大学附属医院住院治疗的911例下呼吸道感染成人患者(＞18岁)的痰液标本,分别进行呼吸道病原菌核酸检测和痰培养,比较分析两种检测方法的检出结果...

Distribution characteristics of pathogenic bacteria in lower respiratory tract infection by using nucleic acid isothermal amplification detection

Objective To investigate the clinical application value of loop mediated isothermal amplification (LAMP) in the detection of respiratory tract pathogens. Methods The sputum specimens were collected from 911 adult patients (>18 years old) with lower respiratory tract infection in the Affiliated Hospital of Xuzhou Medical University from March 2018 to April 2019, and the nucleic acid detection and sputum culture of respiratory pathogens were carried out respectively, and the detection results of the two methods were compared and analyzed. Results By using LAMP method, the detection rates of Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Haemophilus influenzae in 911 sputum samples were 11.2%, 28.8%, 51.8%, 32.6% and 15.9% respectively, which were higher than 4.0%, 8.2%, 8.6%, 17.7% and 7.8% by using the traditional sputum culture method (P<0.05). Conclusion The main pathogens of lower respiratory tract infection detected by LAMP method were mixed positive rate, and the mixed positive rate of Escherichia coli, Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Acinetobacter baumannii were higer. Compared with the traditional sputum culture, LAMP method has the advantages of rapid, high specificity and wide coverage of pathogens.