人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响

目的 探讨人骨髓间充质干细胞(human bone marrow mesenchymal stem cells,hBM-MSCs)对舌鳞癌细胞系Cal-27侵袭的影响及作用机制。方法 分别培养hBM-MSCs和Cal-27,倒置显微镜下观察细胞形态。hBM-MSCs和Cal-27共培养,获得共培养后的Cal-27细胞。划痕实验观察Cal-27的迁移面积;Transwell迁移及侵袭实验观察Cal-27的迁移及侵袭细胞数目,绘制条形图。使用荧光定量PCR技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物E-cadherin、twist、slug、snail、MMP-2、MMP-9基因表达的影响;使用Western 免疫印迹技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物MMP-2、MMP-9蛋白表达的影响。采用SPSS 19.0软件包对数据进行统计学分析。结果 hBM-MSCs可促进Cal-27的侵袭,伴随E-cadherin下调,twist、slug、snail、MMP-2、MMP-9基因表达上调及MMP-2、MMP-9蛋白表达上调。结论 hBM-MSCs促进Cal-27细胞的侵袭作用,可能与其上调Cal-27细胞侵袭作用相关肿瘤标志物的表达有关。

Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27

PURPOSE: To investigate the effect of human bone marrow mesenchymal stem cells(hBM-MSCs) on invasion of tongue squamous cell carcinoma cell line Cal-27 and its mechanism. METHODS: hBM-MSCs and Cal-27 were cultured respectively, and the morphology of the cells was observed under an inverted microscope. The co-cultured Cal-27 cells were obtained by co-culture of hBM-MSCs and Cal-27. The migration area of Cal-27 was observed by scratch test;transwell migration and invasion experiments were performed to observe migration and invasion of Cal-27, and a bar graph was then drawn. Fluorescence quantitative PCR was used to observe the effect of hBM-MSCs on gene expression of the tumor markers E-cadherin, twist, slug, snail, MMP-2 and MMP-9. Western blot was used to observe the effect of hBM-MSCs on protein expression of MMP-2 and MMP-9, related to the invasion of Cal-27. SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: Under the influence of hBM-MSCs, the invasion of Cal-27 was promoted, accompanied by down-regulation of E-cadherin, up-regulation of twist, slug, snail, MMP-2, MMP-9 and upregulation of MMP-2 and MMP-9 expression. CONCLUSIONS: hBM-MSCs can promote invasion of Cal-27 cells, which may be related to up-regulation of the expression of tumor markers related to invasion of Cal-27 cells.