miRNA132在抑郁症患者及慢性不可预知温和应激抑郁模型大鼠中的表达

目的 观察首发抑郁症患者外周血及慢性不可预知温和应激(CUMS)抑郁模型大鼠血液和前额叶皮层中miRNA132的表达水平。方法 以2017年3月至2018年5月在杭州市第七人民医院精神科门诊治疗的首发抑郁症患者(抑郁组)41例及同期招募的健康志愿者(对照组)31例为研究对象,采用汉密尔顿抑郁量表(HAMD)评分评估抑郁症患者严重程度。雄性SD大鼠24只,采用配对比较法随机分为对照组和CUMS组,每组12只;分别用糖水偏好实验和强迫游泳实验检测大鼠抑郁样行为,ELISA法检测大鼠血浆皮质酮水平。采用实时荧光定量PCR法检测受试者外周血白细胞中和大鼠血液及前额叶皮层中miRNA132表达水平。结果 抑郁症患者外周血白细胞miRNA132水平为2.37±0.36,明显高于对照组的1.34±0.16(t= 2.355,P=0.0213);抑郁症组患者外周血白细胞miRNA132表达水平与HAMD17得分呈显著正相关(P=0.0004,r_s=0.5303,n=41)。CUMS组大鼠不动时间为(72.67±2.95)s,明显多于对照组的(40.00±5.49)s(t=2.366,P=0.0395);蔗糖消耗为(55.67±6.42)%,明显低于对照组的(98.21±1.28)%(t=6.502,P<0.0001);血浆皮质酮水平为(1396.0±254.9)nmol/L,明显高于对照组的(557.3±158.4)nmol/L(t=2.795,P=0.0190)。CUMS组大鼠血液中白细胞和前额叶皮层中的miRNA132表达水平分别为2.32±0.88和2.80±0.76,明显高于对照组的1.18±0.36(t=2.273,P=0.0463)和0.99±0.23(t=2.553,P=0.0287)。结论 临床抑郁症患者外周血miRNA132表达趋势与CUMS抑郁模型大鼠miRNA132表达趋势一致。CUMS抑郁模型大鼠血液中miRNA132表达与前额叶皮层中表达也一致,血液miRNA132表达可在一定程度上反映前额叶皮层的miRNA132表达变化趋势。

Expression Levels of miRNA132 in Depression Patients and Chronic Unpredictable Mild Stress Rats

Objective To investigate the expression levels of miRNA132 in patients with the first-episode major depressive disorder(MDD) and in chronic unpredictable mild stress(CUMS)rats.Methods Forty-one first-episode MDD patients(MDD group)were recruited from the outpatient departments of Hangzhou Seventh People’s Hospital between March 2017 and May 2018,and 31 healthy volunteers(control group)were recruited.The patients’ severity of symptoms was assessed with HAMD17.In addition,24 male SD rats were equally assigned into control group and CUMS group.The depression-like behaviors of rats was detected by sucrose preference test and forced swimming test.Plasma corticosterone levels of rats were assayed by ELISA.The expression levels of miRNA132 in the blood or prefrontal cortex were detected by quantitative real-time PCR.Results The expression level of miRNA132 in peripheral blood was significantly higher in MDD group(2.37±0.36)than in control group(1.34±0.16)(t=2.355,P=0.0213),and there was a positive correlation between miRNA132 levels and the HAMD17 score in MDD group(P=0.0004,r_s=0.5303,n=41).The immobility time of CUMS group (72.67±2.95)s] was significantly longer than that of control group (40.00±5.49)s] in forced swim test(t=2.366,P=0.0395).The sucrose intake of CUMS group (55.67±6.42)%] was significantly lower than that of control group (98.21±1.28)%] in sucrose preference test(t=6.502,P<0.0001).The plasma corticosterone level in CUMS group (1396.0±254.9)nmol/L] was significantly higher than that of control group (557.3±158.4)nmol/L](t=2.795,P=0.0190).The miRNA132 levels in blood(2.32±0.88)and prefrontal cortex(2.80±0.76)of CUMS rats were significantly higher than those 1.18±0.36(t=2.273,P=0.0463)and 0.99±0.23(t=2.553,P=0.0287),respectively] of control group.Conclusions The expression trend of miRNA132 in peripheral blood is consistent between MDD patients and CUMS rats.In CUMS rats,the expression of miRNA132 in blood is also consistent with that in prefrontal cortex.The expression of miRNA132 in blood may reflect the change trend of miRNA132 expression in prefrontal cortex.