体外无血清小鼠胚胎种植模型的建立

目的 观察小鼠胚胎的体外发育及种植过程。方法 采用全组蜕膜细胞培养方式进行体外培养,然后移入孕４ｄ的小鼠胚胎,建立无血清联合培养体系,对胚胎和蜕膜细胞的生长情况进行显微观察。结果 小鼠胚胎在体外无血清培养体系中能够顺利孵化、粘附、种植及外延生长,并形成外胎盘锥和羊膜囊;蜕膜细胞的形态在４ｄ与小鼠胚胎的共同培养时限内保持完好。结论 蜕膜细胞是理想的培养的饲养细胞,本实验方法具有简单、实用和可靠的特点

Establishment of in vitro Serum free Embryo Implantation Model

Objective To observe the growth and implantation of mouse embryos in vitro. Methods In order to establish a serum free coculture system, the whole components of decidual tissue was collected and cultured in vitro. Then 4 day mouse embryos were added to the well developed decidual cells, and the following changes of the above system were closely observed under microscope. Results Mouse embryos were successfully hatched, adhered, implanted and outspreaded under the coculture system. The extraplacental cone and amniotic sac were formed as well. Decidual cells kept stable during the following 4 day coculture. Conclusions Decidual cell is an ideal feeder cell for coculture. Our results showed that the modified method of decidual cell culture and the establishment of implantation model were simple, practical and reliable.