HPLC法测定复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量

目的：建立高效液相色谱法测定复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量。方法：采用HPLC法,色谱柱：Sunfire C18（250 mm ×4.6 mm,5μm ）,流动相：乙腈-水（60∶40）,流速1.0 ml·min-1,检测波长：240 nm,柱温：30℃,进样量：20μl。结果：甲氧沙林和醋酸地塞米松分别在12.71~101.68 mg·L-1（r =0.9999）和10.03~80.25 mg·L-1（r =0.9997）范围内线性关系良好,平均回收率分别为99.32%（RSD=0.11%,n=9）和99.63%（RSD=0.20%,n=9）。结论：该法简便、准确、重复性好,可用于复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量测定。

Determination of Methoxsalen and Dexamethasone Acetate in Compound Methoxsalen Lotions by HPLC

Objective：To establish an HPLC method for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions. Methods：An HPLC method was used. The determination was performed on a Sunfire C18 （250 mm× 4. 6 mm,5μm ） column with the mobile phase of acetonitrile-water （ 60∶40 ） at the flow rate of 1. 0 ml·min-1 . The column temperature was kept at 30℃, the detection wavelength was set at 240nm and the injection volume was 20μl. Results：Methoxsalen and dexamethasone acetate showed good linear relationship within the range of 12.71-101.68 mg·L-1（r=0.999 9） and 10.03-80.25 mg·L-1（r=0. 999 7） with the average recovery of 99. 32%（RSD=0. 11%, n=9 ） and 99. 63%（RSD=0. 20%,n=9） , respectively. Conclu-sion：The method is simple, accurate and repeatable, and suitable for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions.