地塞米松对小梁细胞粘附及吞噬功能的影响

目的：探讨地塞米松对小梁细胞的粘附及吞噬功能的影响。方法：不同浓度地塞米松(10^-7M，10^-6M，10^-5M)处理体外培养的牛眼小梁细胞3d，消化、漂洗后加入用不同的细胞外基质(Extracellular matrix，ECM)包被的培养板中，37℃孵育90min后．采用甲基噻唑基四唑(Methyl thiazolyl tetrazolium，MW)法测定各组吸光度值反应粘附细胞的量；另外，以乳胶微球为标记，观察不同浓度的地塞米松对小梁细胞吞噬功能的影响。结果：与对照组比较，三种浓度的地塞米松均对小梁细胞与ECM粘附有抑制作用．且呈浓度依赖性；对小梁细胞的吞噬功能亦呈现浓度依赖性抑制作用。结论：地塞米松对小梁细胞的粘附及吞噬功能有抑制作用，抑制小梁细胞的粘附及吞噬功能可能是皮质类固醇性青光眼的发病原因之一。

Effect of Dexamethasone on Adhesion and Phagocytosis of Bovine Trebacular Meshwork Cells

PURPOSE: To study the effect of dexamethasone on adhesion and phagocyosis of bovine trebacular cells (BTC). METHODS: After treated with different concentrations of dexamethasone (10(-7) M, 10(-6) M, 10(-5) M) for three days, BTC were added to 96-well plates coated with various extracellular matrix (ECM) and incubated for 90 minutes at 37 degrees C, Methyl thiazolyl tetrazolium (MTT) assay were used to evaluate the amount of the cells attach to ECM; In addition, using latex micropheries 0.8 microm in diameter as markers,we investigated the effect of dexamethasone on phagocytosis of BTC. RESULTS: All groups of Dexamethasone can inhibit BTC attach to various ECM and in a dose-depend manner; the phagocytosis of BTC were also inhibited by three concentrations of dexamethasone and in a dose-depend manner. CONCLUSIONS: Dexamethasone can inhibit adhesion and phagocytosis of BTC, it may be related with pathogenasis of glucocorticoid induced glaucoma.