| 曲古抑菌素A对乳腺癌细胞株MDA-MB-231增殖的影响 |
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| 引用本文： | 聂建云,刘馨,黄云超. 曲古抑菌素A对乳腺癌细胞株MDA-MB-231增殖的影响[J]. 山东医药, 2009, 49(43): 22-23 |
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| 作者姓名： | 聂建云 刘馨 黄云超 |
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| 作者单位： | 昆明医学院第三附属医院,昆明,650118;昆明医学院第三附属医院,昆明,650118;昆明医学院第三附属医院,昆明,650118 |
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| 基金项目： | 中国人事部留学人员科技活动项目择优资助启动基金,云南省科技厅应用基础研究项目 |
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| 摘 要： | 目的探讨曲古抑菌素A（TSA）对乳腺癌细胞株增殖的影响。方法培养乳腺癌细胞株MDA-MB-231,用组不同浓度TSA分别作用于细胞,于24、48、72、96h用MTT比色法观察细胞生长情况,流式细胞术检测S期细胞的比例和周期素（Cyclin）D1、A2表达。结果72h内和4μmol／L的浓度范围内,乳腺癌细胞生长受到抑制,并有一定的时效关系;TSA作用后周期素Cyclin A2表达下降,Cyclin D1表达上升（P均〈0．05）。结论TSA能抑制乳腺癌细胞生长,周期素Cyclin D1、Cyclin A2参与了抑制剂对细胞增殖周期的调控。
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| 关 键 词： | 乳腺癌 曲古抑菌素A 细胞增殖 |
Effect of Trichostatin A on cell proliferation of breast cancer cell line MDA-MB-231 |
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| NIE Jian-yun,LIU Xin,HUANG Yun-chao. Effect of Trichostatin A on cell proliferation of breast cancer cell line MDA-MB-231[J]. Shandong Medical Journal, 2009, 49(43): 22-23 |
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| Authors: | NIE Jian-yun LIU Xin HUANG Yun-chao |
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| Affiliation: | (The Third Affiliatied Hospital of Kunming College, Kunming 650118 , P. R. China) |
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| Abstract: | Objective To investigate the effect of Trichostatin A （ TSA ） on the cell proliferation of breast cancer cell line. Methods The breast cancer cell line MDA-MB-231 was cultured and treated with TSA in different concentrations. The cell growth was detected by M＇Iq＂ assay 24,48 ,72 and 96 hours later,the proportion of S-phase cell and expression of Cyclin A2,Cyclin D1 were measured by the flow eytometry. Results The cell growth was inhibited in time-effect manner 24-72 hours after the treatment of TSA in concentration of 1 - 4 lxInol/L. The expression of Cyclin A2 decreased, the expression of Cyelin D1 increased after the treatment of TSA. Conclusions TSA can inhibit the cell growth of breast cancer , Cyclin A2 and Cyelin D1 involes in the regulation of cell proliferation cycle. |
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| Keywords: | breast neoplasm Trichostatin A cell proliferation |
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