| 葛根素对大鼠脑缺血再灌注细胞间粘附分子-1表达的影响 |
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| 引用本文： | 杨黎,何世银.葛根素对大鼠脑缺血再灌注细胞间粘附分子-1表达的影响[J].中国中西医结合杂志,2003(Z1). |
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| 作者姓名： | 杨黎 何世银 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院 （武汉430022） |
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| 摘 要： | 目的:探讨葛根素抗缺血再灌注损伤的机制是否与其抑制细胞间粘附分子-1(ICAM-1)表达相关。方法:采用线栓法制备大鼠局灶性大脑中动脉栓塞(MCAO)模型,以免疫组化的方法观察葛根素对大鼠脑缺血再灌注后血管内皮细胞 ICAM-1蛋白表达的影响,并以比色法观察其对脑组织中髓过氧化物酶(MPO)活性的影响。结果:葛根素能有效地抑制再灌注各时间点的 ICAM-1蛋白的过量表达,并抑制 MPO的活性(P<0.05或 P<0.01)。结论:葛根素可下调缺血再灌注后脑组织中 ICAM-1蛋白的表达,并可抑制MPO 的活性。
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| 关 键 词： | 葛根素 缺血再灌注损伤 细胞间粘附分子-1 髓过氧化物酶 |
Effect of Puerarin on Expression of Intercellular Adhesion Molecule-1 in Cerebral Ischemia/Reperfusion Rats |
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| Authors: | YANG Li; HE Shi-yin |
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| Institution: | YANG Li; HE Shi-yin Union Hospital of Tongji Medical College; Huazhong Science and Technology University; Wuhan(430022); |
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| Abstract: | Objective:To investigate whether the anti-ischemia/reperfusion effect of puerarin is related to its ifihibition on the expression of intercellular adhesion molecule-1 (ICAM-1).Methods:The focal cerebral ischemia reperfusion model was established by means of thread ligation caused middle cerebral artery occlusion (MCAO) and embolism,ICAM-1 was determined and localized by immunohis- tochemistry method and the effect on activity of myeloperoxidase enzyme(MPO) in brain tissue was measured with colorimetry metho... |
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| Keywords: | puerarin ischemia/reperfusion injury intercellular adhesion molecule-1 myeloperoxidase |
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