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| 灵芝孢子粉碱提多糖对小鼠巨噬细胞的免疫调节作用 | |
| 引用本文： | 唐庆九,张劲松,潘迎捷,Werner Reutter,樊华. 灵芝孢子粉碱提多糖对小鼠巨噬细胞的免疫调节作用[J]. 细胞与分子免疫学杂志, 2004, 20(2): 142-144 |
| 作者姓名： | 唐庆九 张劲松 潘迎捷 Werner Reutter 樊华 |
| 作者单位： | 1. 柏林自由大学分子生物与生物化学研究所,德国,柏林;上海农业科学院食用菌研究所,上海,201106 2. 上海农业科学院食用菌研究所,上海,201106 3. 柏林自由大学分子生物与生物化学研究所,德国,柏林 |
| 基金项目： | 德国“BundesministriumfurForschungundTechnologie,Bonn”科研基金资助 (No .SFB36 6 ) |
| 摘 要： | 目的 :研究灵芝孢子粉碱提多糖 (LZSBS)对小鼠巨噬细胞的激活作用。方法 :用灵芝孢子粉碱提多糖刺激体外培养的小鼠巨噬细胞 ,用ELISA法检测巨噬细胞分泌至培养基中的TNF α和IL 1β的含量 ;用Griess法检测培养上清中NO的含量。小鼠巨噬细胞对乳胶颗粒的吞噬率用显微镜计数。结果 :经灵芝孢子粉碱提多糖刺激后 ,小鼠巨噬细胞变大 ,颜色加深 ,并能显著刺激巨噬细胞分泌TNF α和IL 1β ,并产生大量的NO。小鼠巨噬细胞对乳胶颗粒的吞噬功能也明显的增强。结论 :灵芝孢子粉碱提多糖对小鼠巨噬细胞具有明显的激活作用 |
| 关 键 词： | 灵芝孢子粉 多糖 细胞因子 巨噬细胞 小鼠 |
| 文章编号： | 1007-8738(2004)02-0142-03 |
| 修稿时间： | 2003-06-02 |
Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum |
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| TANG Qing-jiu ,,ZHANG Jin-song ,,PAN Ying-jie ,Werner Reu tter ,FAN Hua Institute of Molekularbiology and Biochemistry,Free University Berlin,Berli n,Germany, Edible Fungi Institute,Shanghai Academy of Agricultural Scie nces,Shanghai ,China. Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum[J]. Chinese journal of cellular and molecular immunology, 2004, 20(2): 142-144 | |
| Authors: | TANG Qing-jiu ZHANG Jin-song PAN Ying-jie Werner Reu tter FAN Hua Institute of Molekularbiology Biochemistry Free University Berlin Berli n Germany Edible Fungi Institute Shanghai Academy of Agricultural Scie nces Shanghai China |
| Affiliation: | Institute of Molecularbiology and Biochemistry, Free University Berlin, Berlin, Germany. tangqingjiu2003@yahoo.com.cn |
| Abstract: | AIM: To investigate the activation of mouse macrophages by the alkali-extracted polysaccharides from the spore of Ganoderma lucidum (LZSBS). METHODS: The mouse macrophages cultured in-vitro were stimulated by LZSBS. IL-1beta and TNF-alpha in the culture supernatants were detected by ELISA. NO production was detected by Griess assay. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope. RESULTS: The mouse macrophages stimulated by LZSBS increased in volume and darkened in appearance under phase-contrast microscope. LZSBS-activated mouse macrophages secreted IL-1beta and TNF-alpha produced a large amount of NO. The percentage of phagocytosis of latex beads by mouse macrophages was also significantly increased in the presence of LZSBS. CONCLUSION: LZSBS can activate markedly the mouse macrophages. |
| Keywords: | spore of Ganoderma lucidum polysaccharide cytokin e macrophages mouse |
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