人乳头瘤病毒感染和p53基因突变与宫颈癌的关系

目的：探讨人乳头瘤病毒１６ 和１８ 型及抑癌基因ｐ５３ 突变对宫颈的致癌作用以及 Ｈ Ｐ Ｖ 感染与ｐ５３ 基因突变的相关性。方法：采用聚合酶链反应（ Ｐ Ｃ Ｒ） 技术和限制性酶切片段多态性分析（ Ｒ Ｆ Ｌ Ｐ） 技术对３４ 例原发性宫颈癌组织及３０ 例正常宫颈组织 Ｈ Ｐ Ｖ１６ ，１８ 型 Ｄ Ｎ Ａ 及抑癌基因ｐ５３ 的突变进行了检测。结果： Ｈ Ｐ Ｖ１６ ，１８ Ｄ Ｎ Ａ 在宫颈癌的总阳性率为６４７ ％ （２２／３４） ，正常宫颈组织只有６７ ％ 阳性，８例宫颈癌组织出现ｐ５３ 基因第６ 外显子突变，其中２ 例为 Ｈ Ｐ Ｖ１６ Ｄ Ｎ Ａ 阳性、１ 例 Ｈ Ｐ Ｖ１８ Ｄ Ｎ Ａ 阳性。结论：宫颈癌的发病与 Ｈ Ｐ Ｖ 感染及ｐ５３ 基因突变有关，宫颈癌组织中ｐ５３ 基因突变与 Ｈ Ｐ Ｖ 感染无关。

The study of relationship between human papilloma virus infection and p53 gene mutation in cervical carcinoma

Objective:To study the role of human papilloma virus (HPV) infection and p53 gene mutations in the oncogenesis of cervical cancer and to clarify the association between p53 gene mutation and the presence of HPV DNA.Methods:34 primary cervical carcinomas and 30 normal cervical specimens were examined for the presence of HPV16,18 DNA by PCR,the p53 gene(exons 6,7) was analyzed by PCR RFLP.Results:HPV16,18 were detected in 64.7%(22/34) of the tumors.HPV18 DNA was positive in 6.7%(2/30) of normoral cervical specimens.However no HPV16 DNA was found in normal cervical tissues.Mutatoins of p53 gene (exon 6) were detected in 8 of 34 tumors.Two of the cases with p53 mutation were positive for HPV16 DNA and one positive for HPV18 DNA.Conclusions:Cervical carcinoma is strongly associated with HPV16 and HPV18 infection,p53 gene mutation is independent of HPV infection.