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| 天然抗角蛋白自身抗体产生细胞的检测 | |
| 引用本文： | 邢影,李巍,付萌,田蓉,韩骅,王刚,刘玉峰. 天然抗角蛋白自身抗体产生细胞的检测[J]. 中国皮肤性病学杂志, 2006, 20(3): 138-140 |
| 作者姓名： | 邢影 李巍 付萌 田蓉 韩骅 王刚 刘玉峰 |
| 作者单位： | 1. 第四军医大学西京医院皮肤科,陕西,西安,710032 2. 第四军医大学遗传与发育生物学教研室,陕西,西安,710032 |
| 基金项目： | 中国科学院资助项目;军队重点项目 |
| 摘 要： | 目的明确产生天然抗角蛋白自身抗体(anti-keratin autoantibody,AK auto Ab)的B细胞的类群和定位。方法取SPF级C57BL/6小鼠的腹腔细胞及脾细胞,进行体外培养,采用ELISA方法分析培养细胞分泌AK auto Ab的水平,ELISPOT方法分析分泌AK auto Ab的细胞数。结果腹腔细胞中分泌AK auto Ab的细胞数远多于脾细胞,其分泌的抗体滴度也显著高于脾细胞。结论AK auto Ab主要来源于腹腔B细胞,B1细胞可能是产生AK auto Ab的主要细胞。 |
| 关 键 词： | 角蛋白 天然抗角蛋白自身抗体 ELISPOT |
| 文章编号： | 1001-7089（2006）03-0138-03 |
| 收稿时间： | 2005-04-27 |
| 修稿时间： | 2005-04-272005-12-29 |
Detection of Natural Anti-keratin Autoantibody Producing Cells |
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| XING Ying, LI Wei, FU Meng,et al. Detection of Natural Anti-keratin Autoantibody Producing Cells[J]. The Chinese Journal of Dermatovenereology, 2006, 20(3): 138-140 | |
| Authors: | XING Ying LI Wei FU Meng et al |
| Abstract: | Objective To clarify the subsets and location of B cells producing natural anti-keratin autoantibody(anti-keratin autoantibody,AK auto Ab).Methods Cells from spleen and peritoneal cavity of C57BL/6 mice were cultured in vitro,and ELISPOT was utilized to detect and calculate the cells that can identify keratin,and ELISA was applied to measure anti-keratin IgM level in culture supernatant.Results Number of Cells producing anti-keratin antibody in peritoneal cavity are greatly higher than those in spleen.And anti-keratin antibody level in the supernatant of cultured peritoneal cell was much higher than that of spleen cells.Conclusion Anti-keraitn autoantibody mainly originate from peritoneal cavity B cells,and B1 cells in the peritoneal cavity maybe the main source of anti-keraitn autoantibody. |
| Keywords: | ELISPOT |
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