双膦酸盐对骨形成蛋白诱导骨的作用

目的：研究双膦酸盐对骨形成蛋白（BMP）诱导骨的作用。方法：42只大白鼠背部植入BMP，诱导出异位骨后，将大白鼠分成实验组和对照组。实验组在BMP植入后第3～7周，每周腹腔注射双膦酸盐（YM175）3次, 剂量1 μg·kg^-1·d ^-1。对照组按同样的方式给予等量的生理盐水。在BMP植入第3、4、7和10周，将BMP诱导骨取出，采用显微放射照片（CMR）和HE染色方法观察双膦酸盐对骨形成蛋白诱导骨的作用。结果：BMP诱导骨3周时（即双膦酸盐未投药前），在BMP植入体周边形成编织骨，大量破骨细胞出现在新生骨组织表面。在4周时，实验组和对照组新生骨组织均向BMP植入体内生长。在10周时，对照组骨细胞变小，实验组可观察到有规律地排列的板层状骨的特征。结论：双膦酸盐对骨形成蛋白诱导骨破骨骨吸收有明显的抑制作用。

Effects of bisphosphonate on ectopic bone induced by bone morphogenetic protein

Objective To determine the effects of bisphosphonate(YM175) on the quality of bone morphogenetic protein(BMP)-induced bone.Methods Bisphosphonate was administered to rats in which subcutaneous ectopic bones were induced by recombinant human BMP-2.Bisphosphonate(YM175) 1 μg·kg~(-1)·d~(-1) was administered to rats carrying the BMP-induced bones three times per week,from the 3rd to 7th week after BMP implantation (bisphosphonate group).Aliquots of phosphate buffered saline were administered in the same protocol without bisphosphonate in the control group.At the 3rd,4th,7th or 10th week,the BMP-induced bones were removed and observed by contact microradiography(CMR) and hematoxylin and eosin(H&E)staining.Results By(3 weeks) when bisphosphonate started to be administered,woven bones formed in the periphery of the BMP pellets.At the 4th week,in both the bisphosphonate and control groups,bone formation advanced inward. During the 10 weeks,bone marrow was formed and the characteristics of lamellar bones,in which the lanunae of the small osteocytes were regularly arranged,were noted in the control group.Conclusion These findings suggest that bone resorption can be inhibited by the bisphosphonate.