乌鳖颗粒对免疫性卵巢早衰小鼠CD4^＋／CD8^及Fas、FasL蛋白表达的实验研究

目的：观察乌鳖颗粒对免疫性卵巢早衰小鼠CD4^＋／CD8^及Fas、FasL蛋白表达的影响。方法：采用雌性BALB／c小鼠皮下多点注射小鼠透明带多肽，建立免疫性卵巢早衰模型。将造模成功的小鼠随机分为模型组、己烯雌酚组、六味地黄丸组、乌鳖颗粒大、中、小剂量组。另设正常组和佐剂组，共灌胃给药4周。实验末应用免疫组化法测定脾脏CD4^＋、CD8^，计算CD4^＋／CD8^的比值。应用免疫组化法测定卵巢Fas、FasL蛋白的表达。结果：模型组小鼠脾脏CD4^＋／CD8^比值明显高于正常组和佐剂组（P〈0．05）。乌鳖颗粒各剂量组小鼠脾脏CD4^＋／CD8^比值明显低于模型组（P〈0．05，P〈0．01）。模型组小鼠卵巢Fas蛋白表达低于正常组，Fas—L蛋白表达明显高于正常组，卵巢Fas、Fas—L蛋白表达和Fas／Fas—L比值与正常组比较具有显著性差异（P〈0．05，P〈0．01）。乌鳖颗粒大剂量组和中剂量组小鼠Fas蛋白表达明显高于模型组，（P〈0．05，P〈0．01）。乌鳖颗粒各剂量组Fas—L蛋白表达明显低于模型组（P〈0．05，P〈0．01）。乌鳖颗粒大剂量组、中剂量组小鼠Fas／Fas—L比值与模型组比较具有显著性差异（P〈0．01）。结论：乌鳖颗粒对免疫性卵巢早衰小鼠具有调节CD4^＋／CD8^比值和Fas／FasL系统的作用。

Effects of Wu Bie Granules on CD4^＋/CD8^ and Fas/FasL Protein Expression of Mice with Immune Premature Ovarian Failure

Objective： Observing the effects of Wu Bie granules on CD4^＋/CD8^ and Fas/FasL protein expression of mice with immune premature ovarian failure. Methods ： The zona pellucida 3 of mice was regarded as antigen, immune POF models of Bulb/c female mice were established by multi - point injection of the antigen. The successful model of the mice were randomly divided into model group, diethylstilbestrol group, Liu Wei Di Huang Wan Group, Wu Bie granules of large, medium,low dose group, separate normal and adjuvant group. Each group began to be given the corresponding medicine for 4 weeks. End of the experiment, T lymphocyte cell subsets of the spleen were detected by immunohistochemistry; detected Fas, FasL protein expression of ovarian were also detected by immunohistochemistry. Results：The CD4^＋/CD8^ of Model group was significantly higher than that of the normal group and adjuvant group, showing significant difference （ P 〈 0. 05 ）. The CD4^＋/CD8^ of Wu Bie granules was significantly lower than that of the model group, compared with the model group, it was significant different（ P 〈 0.05 ,P 〈 0.01 ）. Fas protein expression of model group was lower than that of normal group ;Fas - L protein expression was significantly higher than that of the normal group. Compared with the normal group Fas, Fas - L protein expression was significant difference （ P 〈 0.05,P 〈 0.01 ）. Wu Bie granules （ large, medium dose） was significantly higher than that of Fas protein expression in model group. Compared with the model group it and significant difference （ P 〈 0.05, P 〈 0.01 ）. The expression of Fas - L protein of Wn Bie granules was significantly lower than that of the model group, compared with the model group it was significant different （P 〈0.05 ,P 〈0. 01 ）. Conclusion：Wu Bie granules can regulate CD4^＋/CD8^ and the Fas / FasL system.