| 腺病毒介导的NDRG2基因对前列腺癌细胞株DU145的影响 |
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| 引用本文： | 高磊,刘学武,刘新平,王禾,于垂恭,赵毅,武国军. 腺病毒介导的NDRG2基因对前列腺癌细胞株DU145的影响[J]. 实用医学杂志, 2010, 26(7). DOI: 10.3969/j.issn.1006-5725.2010.07.007 |
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| 作者姓名： | 高磊 刘学武 刘新平 王禾 于垂恭 赵毅 武国军 |
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| 作者单位： | 1. 第四军医大学西京医院泌尿外科,西安市,710032
2. 第四军医大学基础部生物化学与分子生物学教研室,西安市,710032 |
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| 摘 要： | 目的:探讨腺病毒介导的N-myc下游调节基因2(NDRG2)基因对前列腺癌细胞株DU145增殖抑制及诱导其凋亡的作用.方法:以携带人NDRG2基因的腺病毒载体转染体外培养的前列腺癌细胞株DU145.采用westernblot检测目的基因的表达,通过细胞生长实验、平板克隆实验检测NDRG2对DU145细胞增殖能力的影响.流式细胞仪检测转染前后细胞凋亡的情况:光镜观察细胞形态学的改变.结果:DU145细胞经腺病毒转染后westernblot检测有NDRG2蛋白(40 kD)特异表达.MTT比色及平板克隆实验结果显示NDRG2对DU145细胞生长有明显抑制作用(P<0.05).流式细胞检测结果显示Ad-NDRG2组凋亡率明显高于对照组及Ad-LacZ组,差异有显著性(P<0.05).与对照组及Ad-LacZ组比较,光镜下观察可见Ad-NDRG2转染的细胞生长状态明显变差,细胞变圆,边缘模糊.结论:通过腺病毒载体使细胞表达NDRG2基因,可以明显抑制DU145细胞的生长和增殖,并可诱导细胞凋亡.
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| 关 键 词： | 前列腺肿瘤 NDRG2基因 腺病毒科 凋亡 |
Adenovirus-mediated NDRG2 gene expression in prostate cancer cell line DU145 |
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| GAO Lei,LIU Xue-wu,LIU Xin-ping,WANG He,YU Chui-gong,ZHAO Yi,WU Guo-jun. Adenovirus-mediated NDRG2 gene expression in prostate cancer cell line DU145[J]. The Journal of Practical Medicine, 2010, 26(7). DOI: 10.3969/j.issn.1006-5725.2010.07.007 |
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| Authors: | GAO Lei LIU Xue-wu LIU Xin-ping WANG He YU Chui-gong ZHAO Yi WU Guo-jun |
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| Affiliation: | GAO Lei,LIU Xue-wu,LIU Xin-ping,WANG He,YU Chui-gong,ZHAO Yi,WU Guo-jun.Department of Urology,Xijing Hospital,Department of Biochemistry , Molecular Biology,the Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | Objective To investigate the effect of NDRG2 gene over-expression on the cell proliferation and apoptosis of prostate cancer DU145 cell.Methods Recombinant adenovirus was used to infected prostate cancer cell line DU145 in vitro.NDRG2 protein expression in cells infected with Ad-NDRG2 or Ad-LacZ was detected using Westernblot assay.MTT and colony formation assays were performed to determine cell proliferation.Cell apoptosis was detected by flow cytometry.Cells morphology was observed under the light microsc... |
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| Keywords: | Prostatic neoplasms Adenoviridae NDRG2 gene Apoptosis |
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