视网膜前膜内相关炎性细胞因子的免疫组化研究

目的 研究增生性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅ ｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）的视网膜前膜（ｅｐｉｒｅｔｉｎａｌ ｍｅｍｂｒａｎｅｓ，ＥＲＭ）中炎性细胞因子白细胞介素－１β（ｉｎｔｅｒｌｅｕｋｉｎ－１β，ＩＬ－１β）、白细胞介素－６（ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）、白细胞介素－８（ｉｎｔｅｒｌｅｕｋｉｎ－８，ＩＬ－８）和肿瘤坏死因子－α（ｔｕｍｏｒ ｎｅｃｒｏｓｉｓ ｆａｃｔｏｒ－α，ＴＮＦ－α）的表达及其作用。方法 用免疫组织化学方法对１９例复杂性视网膜脱离行玻璃体切割术时剥离的视网膜前膜进行观察。结果 ＩＬ－１β、ＩＬ－６、ＩＬ－８和ＴＮＦ－α分别在９、１２、１１和１５例视网膜前膜中表达，尤其以细胞外基质中明显，４种细胞因子在４例膜中同时表达，仅有１例膜中人表达ＩＬ－６。

Immunohistochemical study of related inflammatory cytokines in epiretinal membrane

Objective　To investigate the expression and role of inflammatory cytokines like interleukin-1β(IL-1β)、interleukin-6(IL-6)、interleukin-8(IL-8) and tumor necrosis factor-alpha(TNF-α)in epiretinal membranes(ERM)of eyes with proliferative vitreoretinopathy(PVR).Methods　Nineteen epiretinal membranes were obtained from eyes undergoing vitrectomy for retinal detachment complicated with PVR and observed by immunohistochemical methods.Results　Expression of IL-1β、IL-6、IL-8 and TNF-α were observed in 9、12、11and 15 membranes respectively, with positive staining mostly in the extracellular matrix of epiretinal membranes. Only one membrane showed positive to IL-6 intracellularly, expression for all the cytokines simultaneously in 4 membranes.Conclusion　The findings indicate that cytokine-mediated pathways of inflammation are involved in the pathogenesis of PVR.