成年大鼠脊髓完全性横断后星形胶质细胞的时空分布及其变化

本研究以成年大鼠脊髓完全性横断模型研究反应性胶质细胞的时空分布和变化。将30只成年Wistar大鼠随机分为5组:正常对照组,T9横断伤1周、2周、4周和8周组,每组6只。利用免疫组织化学方法及图像分析系统对各组动物脊髓内星形胶质细胞的时空分布和变化进行观察和分析。结果显示:脊髓横断组胶质纤维酸性蛋白(GFAP)阳性的星形胶质细胞数目较正常对照组明显增加(P<0.05);距损伤近侧端较距损伤远侧端的GFAP阳性星形胶质细胞数目增加显著(P<0.05);脊髓横断组髓磷脂碱性蛋白(MBP)阳性的少突胶质细胞数目的时间及空间分布与正常对照组相比无统计学差异(P>0.05)。实验结果提示,星形胶质细胞是胶质瘢痕的主要成分,而少突胶质细胞在瘢痕形成过程中并非是反应活跃的成分。

TEMPORAL AND SPATIAL DISTRIBUTIONS AND CHANGES OF ASTROCYTE AFTER A TOTAL TRANSECTION OF THE ADULT RAT SPINAL CORD

To observe the temporal and spatial distributions and changes of reactive glial cells after complete spinal cord injury (SCI) in the rat. Adult Wistar rats(n=30) were randomly divided into five groups: the groups of 1 wk, 2 wk, 4 wk and 8 wk after spinal cord injury and control group (in each group, n=6). Immunohistochemical technique and imaging analysis technique were used to detect the temporal and spatial distributions and changes of the astrocyte in spinal cord in the animals of each group. The results showed that the glial fibrillary acidic protein (GFAP) immunopositive cells were much more in SCI groups than in control group (P<0.05). The number of GFAP immunopositive astrocyte increased with segments adjacent to the injury site than that in the distal segments (P<0.05). The number of myelin basic protein (MBP) immunopositive oligodendrocytes in SCI groups was not obviously different from that in the control group (P>0.05). The results indicate that astrocytes are the main component of the glial scar, oligodendrocytes are not active element of the scar.