不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹特征相似性研究

目的 研究不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹图谱的相似性,为瓜蒌皮与瓜蒌子的合理配伍比例提供理论依据.方法 采用高效液相色谱法建立30:70、35:65、40:60、45:55、50:50、55:45共6种比例的瓜蒌皮-瓜蒌子及瓜蒌的化学指纹图谱及其共有模式,比较不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌化学指纹图谱共有模式间的相似度.结果上述6种比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹图谱共有模式间的相似度分别为0.8808±0.0407、0.8930±0.0236、0.8995±0.0195、0.9033±0.0190、0.9363±0.0082和0.8904±0.0144,其中瓜蒌皮-瓜蒌子比例为50:50时相似度最高.各组与瓜蒌皮-瓜蒌子50:50组比较,差异有统计学意义(P<0.05或0.01).结论 采用指纹图谱技术确定了瓜蒌皮-瓜蒌子的最佳配伍比例,为瓜蒌皮-瓜蒌子的合理配伍提供了理论依据.

Study on the similarity of chemical fingerprints of different compatibility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis

Objective To study the similarity of chemical fingerprints of different proportion of Gualoupi-Gualouzi and Fruc?tus Trichosanthis,so as to provide the theoretical basis for the reasonable proportion of Gualoupi-Gualouzi. Methods The HPLC fin?gerprints and the common patterns of 6 the proportion of the 6 kinds of compatibility(30:70,35:65,40:60,45:55,50:50 and 55:45)of Gualoupi-Gualouzi and Fructus Trichosanthis were established,and the similarity of chemical fingerprints of different compati?bility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis between their common patterns were compared. Results Compared with the common pattern of Fructus Trichosanthis,the similarities of the common patterns of chemical fingerprints of the above 6 kinds of compatibility proportion of Gualoupi-Gualouzi were 0.8808 ± 0.0407,0.8930 ± 0.0236,0.8995 ± 0.0195,0.9033 ± 0.0190,0.9363 ± 0.0082 and 0.8904 ± 0.0144,respectively. Among them,Gualoupi-Gualouzi ratio of 50:50 had the highest similarity,and compared with other groups,the differences were statistically significant(P<0.05 or 0.01). Conclusion The optimum proportion of Gualoupi-Gualouzi can be determined by the fingerprint technology,which provides the theoretical basis for the reasonable proportion of Gualou?pi-Gualouzi.