儿童系统性红斑狼疮患者外周血淋巴细胞表面CD95的表达及临床意义

目的:探讨儿童系统性红斑狼疮(systemic lupus erythematosus,SLE)外周血淋巴细胞表达CD95的特征及与疾病活动性和其他免疫学指标间的关系.方法:使用流式细胞术检测60例SLE患儿和20例对照外周血T淋巴细胞亚群和B淋巴细胞表面CD95的表达,并分析其与SLE疾病活动性以及实验室检查之间的关系.结果:初发SLE患儿外周血中CD4+T细胞表面CD95的表达显著高于对照组,差异有统计学意义(P<0.05);初发SLE患儿外周血中CD19+B细胞表面CD95的表达显著高于健康儿童(P<0.05);CD19+CD95+B细胞的比例和SLE疾病活动性呈正相关(r=0.4,P<0.05);CD4+CD95+T细胞的比例和SLE疾病活动性呈正相关(r=0.3,P<0.05),CD4+CD95+T细胞的比例和外周血抗双链DNA抗体(anti-dsDNA Abs)的水平呈正相关(r=0.2,P<0.05);治疗后SLE患儿外周血中CD19+CD95+B细胞和CD4+CD95+T细胞的比例均有显著下降,差异有统计学意义(P<0.05).结论:儿童SLE患者外周血中淋巴细胞表达CD95的水平显著升高,且与SLE的疾病活动性及血清中抗双链DNA抗体相关,可以作为SLE的评价指标.

Expression and significance of CD95 in periphera1 b1ood lymphocytes of children with new-onset systemic lupus erythematosus

Objective: To investigate the expression of CD95 in peripheral blood lymphocytes of children with systemic lupus erythematosus (SLE) and its relationship with disease activity and other immunological parameters. Methods: Flow cytometry was used to detect the expression of CD95 on T and B lymphocytes in peripheral blood of 60 children with SLE and 20 healthy children, and analyze the relationship between the expression of CD95 and disease activity. Results: The expression of CD95 in CD4+ T cells in peripheral blood of children with SLE was significantly higher than that of healthy children. The expression of CD95 in CD19+ B in peripheral blood of children with SLE was significantly higher than that of healthy children. The proportion of CD4+ CD95+ T cells was positively correlated with SLE disease activity. The proportion of CD19+ CD95+ B cells was positively correlated with SLE disease activity. The proportion of CD4+ CD95+ T cells was positively correlated with the level of anti-double stranded DNA antibody (anti-dsDNA Abs) in peripheral blood. Conclusion: The expression level of CD95 in peripheral blood lymphocytes of children with SLE is significantly increased, and the activity of SLE is correlated with the activity of SLE and anti-double stranded DNA antibody in serum.