GPI、抗-CCP和RF联合检测对诊断类风湿性关节炎的临床意义

目的研究葡萄糖-6-磷酸异构酶(GPI)、环瓜氨酸多肽抗体(抗-CCP)和类风湿因子(RF)联合检测对诊断类风湿性关节炎的临床意义。方法选择2012年1月—2016年12月在本院进行诊治的类风湿性关节炎患者120例为A组,其他自身免疫性疾病患者(包括骨关节病、系统性红斑狼疮、原发性干燥综合征、系统性硬化症以及混合性结缔组织病)100例为B组,以及同期在本院进行体检的健康者100例为C组。采用酶联免疫吸附法检测GPI和抗-CCP,采用免疫比浊法对血清RF进行测定。比较3组研究对象GPI、抗-CCP、RF及联合检测的阳性率以及GPI、抗-CCP、RF与联合检测对类风湿性关节炎的特异度、敏感度、阴性预测值以及阳性预测值。结果 A组GPI、抗-CCP、RF及联合检测的阳性率均明显高于B组以及C组(P0.05);GPI、抗-CCP、RF联合检测对类风湿性关节炎的诊断特异度、敏感度、阴性预测值以及阳性预测值均明显高于单独检测(P0.05)。结论 GPI、抗-CCP和RF在类风湿性关节炎中具有较高的阳性表达率,三者联合检测可以提高对类风湿性关节的检出率。

Clinical significance of combined detection of GPI,anti-CCP and RF in the diagnosis of rheumatoid arthritis

Objective To investigate the clinical significance of combined detection of GPI, anti-CCP and RF in the diagnosis of rheumatoid arthritis.Methods A total of 120 cases of rheumatoid arthritis treated in our hospital during Jan.2012 and Dec.2016 were selected as group A;100 cases of other autoimmune diseases, including osteoarthritis, systemic lupus erythematosus, primary Sjogren`s syndrome, systemic sclerosis and mixed connective tissue disease served as group B;100 healthy controls served as group C.The GPI and anti-CCP were detected with ELISA, and serum RF was measured with immune turbidimetry.The positive rate, specificity, sensitivity, negative predictive value and positive predictive value were compared.Results The positive rate of GPI, anti-CCP, RF and combined detection in group A were significantly higher than those in group B and C (P<0.05).The specificity, sensitivity, negative predictive value and positive predictive value of combined detection of GPI, anti-CCP and RF were significantly higher than those of single detection (P<0.05).Conclusion GPI, anti-CCP and RF have higher positive expression rate in rheumatoid arthritis, and combined detection can improve the detection rate of rheumatoid arthritis.