熄风定眩颗粒对局灶性不完全脑缺血眩晕模型大鼠的治疗作用及机制研究

目的 从大鼠行为学和脑组织能量代谢与氧化应激角度考察熄风定眩颗粒对局灶性不完全缺血眩晕模型大鼠的治疗作用.方法 实验设正常对照组、假手术组、模型组、盐酸氟桂利嗪组(0.5 mg/kg)、银杏叶组(8 mg/kg),以及熄风定眩颗粒0.27、0.54和1.08 g/kg剂量组.分别测定各组大鼠逃避电刺激潜伏期和大鼠前庭神经核血流量,用比色法测定各组脑组织乳酸(LAC)、乳酸脱氢酶(LDH),超氧化物歧化酶(SOD)、丙二醛(MDA)含量.结果 1.与对照组比较,模型组大鼠逃避电刺激潜伏期明显延长(P<0.01),熄风定眩颗粒各剂量组均可不同程度缩短大鼠逃避潜伏期(P<0.01),其中以0.54 g/kg剂量效果最为显著.2.与假手术组比较,模型组右侧大脑前庭核血流明显减少(P<0.01),熄风定眩颗粒各剂量组均可不同程度抑制模型大鼠大脑前庭核血流的减少,其中熄风定眩颗粒0.54和1.08 g/kg剂量组作用显著(P<0.01).3.与假手术组比较,造模后模型组动物右侧大脑乳酸含量、LDH活性明显升高(P<0.01).熄风定眩颗粒各剂量组可显著抑制LDH活性的升高(P<0.01),并不同程度抑制模型大鼠脑内乳酸含量的升高,其中熄风定眩颗粒0.27和0.54 g/kg剂量组作用显著(P<0.01).4.与假手术组比较,造模后模型组动物右侧大脑MDA含量、SOD活性显著升高(P<0.01),熄风定眩颗粒各剂量组可明显抑制模型大鼠脑内MDA含量升高(P<0.01).结论 熄风定眩颗粒可抑制局灶性不完全脑缺血模型大鼠大脑前庭核血流量减少、改善脑内能量代谢,降低氧化应激反应,对局灶性不完全脑缺血眩晕具有治疗作用.

Therapeutic effect and mechanism of Xifeng Dingxuan granules on focal incomplete cerebral ischemia vertigo model rats

Objective To evaluate the effects of Xifeng Dingxuan granules on behavioral changes and brain energy metabo?lism on focal incomplete cerebral ischemia vertigo model rat. Methods Experimental rats were divided into normal control group, sham operation group,model group,flunarizine hydrochloride group(0.5 mg/kg),ginkgo leaf group(8 mg/kg)and Xifeng Dingxuan granule groups(0.27,0.54 and 1.08 g/kg). The drugs were all administered intragastrically. During the experimental period,step-down test was conducted to monitor the escape latency of rats from electrical stimulation,and the blood flows in right brain vestibular nucleus were measured. At the end of the experiment,the levels of the lactate(LAC),lactic acid dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)in brain tissue were determined by colorimetry. Results 1. Compared with the normal control group,the escape latency of rats from electrical stimulation in model group was significantly prolonged(P<0.01). The escape latency was significantly reduced by the treatment of Xifeng Dingxuan granules(P<0.01),and the dosage of 0.54 g/kg was most effec?tive. 2. Compared with the sham operation group,the blood flow of right brain vestibular nucleus in the model group was decreased (P<0.01). The decreased blood flow could be alleviated by the treatment of Xifeng Dingxuan granules and the dosage of 0.27 g/kg was most effective(P<0.01). 3. Compared with sham operation group,the contents of LAC and LDH in the right brain tissue were signifi?cantly increased in the model group(P<0.01). Compared with the model group,the contents of LAC and LDH were decreased signifi?cantly by the treatment of Xifeng Dingxuan granules with the dosage of 0.27 g/kg and 0.54 g/kg(P<0.01). 4. Compared with the sham operation group,the content of MDA and the activity of SOD in the right brain tissue were significantly increased in the model group (P<0.01)and could be alleviated by the treatment with Xifeng Dingxuan granules at each dose(P<0.01). Conclusion The results indicated that Xifeng Dingxuan granules could suppress the decrease of blood flow in vestibular nucleus,improve the brain energy me?tabolism and reduce the oxidative stress response in focal incomplete cerebral ischemia vertigo rat model ,which implicated the possi?ble therapeutic effect of Xifeng Dingxuan granules on the vertigo.