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| 人参皂苷Rg1促智信号转导途径研究 | |
| 引用本文： | 王玉珠,王永胜,楚世峰,陈霁,陈乃宏,张均田. 人参皂苷Rg1促智信号转导途径研究[J]. 中国药理学通报, 2008, 24(6): 740-743 |
| 作者姓名： | 王玉珠 王永胜 楚世峰 陈霁 陈乃宏 张均田 |
| 作者单位： | 中国医学科学院北京协和医学院药物研究所,北京,100050 |
| 摘 要： | 目的探究人参皂苷Rg1(G-Rg1)促智信号转导途径,观察G-Rg1对环腺苷酸(cAMP)、磷酸化cAMP反应元件结合蛋白(p-CREB)和磷酸二酯酶(PDE)的影响。方法正常成年大鼠口服G-Rg120mg.kg-1,连续3d后,分别采用酶联免疫法和免疫组织化学法检测脑区cAMP和p-CREB水平的变化。体外给予G-Rg1后,检测海马匀浆液中PDE活性的变化。结果大鼠口服G-Rg1后,海马组织cAMP水平升高,海马和脑皮质p-CREB增强,G-Rg1降低海马PDE活性,抑制cAMP降解。结论G-Rg1能提高脑内cAMP、p-CREB水平,抑制PDE活性,表明G-Rg1可以通过激活cAMP-PKA-CREB信号通路发挥促智作用。 |
| 关 键 词： | 人参皂苷Rg1 认知功能 cAMP p-CREB PDE 信号转导 |
| 文章编号： | 1001-1978(2008)06-0740-04 |
| 修稿时间： | 2008-01-30 |
Nootropic signal transduction of ginsenoside Rg1 |
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| WANG Yu-zhu,WANG Yong-sheng,CHU Shi-feng,CHEN Ji,CHEN Nai-hong,ZHANG Jun-tian. Nootropic signal transduction of ginsenoside Rg1[J]. Chinese Pharmacological Bulletin, 2008, 24(6): 740-743 | |
| Authors: | WANG Yu-zhu WANG Yong-sheng CHU Shi-feng CHEN Ji CHEN Nai-hong ZHANG Jun-tian |
| Abstract: | Aim To elucidate the nootropic signal transduction of ginsenoside Rg1(G-Rg1) by investigating its effects on cAMP,p-CREB and PDE.Methods Rats were given G-Rg1(20 mg·kg-1) intragastrically for three days,the levels of cAMP and p-CREB were detected with enzyme-linked immuno assay and immunohistochemistry method,respectively.The influence on PDE activity was assayed in hippocampus homogenate in vitro.Results The levels of cAMP in hippocampus and p-CREB in hippocampus and cerebral cortex increased significantly in the rats treated with G-Rg1,and,simultaneously,the activity of PDE was definitely inhibited,leading to raise of cAMP level.Conclusion G-Rg1 may exert its nootropic action through cAMP-PKA-CREB signal pathway. |
| Keywords: | ginsenoside Rg1 cognitive function cAMP p-CREB PDE signal transduction |
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