Differentiation of species and strains among filamentous fungi by DNA fingerprinting |
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| Authors: | Wieland Meyer Anke Koch Claudia Niemann Birgit Beyermann Jörg T. Epplen Thomas Börner |
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| Affiliation: | (1) Institut für Genetik, Fachbereich Biologie, Humboldt-Universität zu Berlin, Invalidenstrasse 43, O-1040 Berlin, Federal Republic of Germany;(2) Industrieforschungszentrum für Biotechnologie GmbH, Alt Stralau 52-54, O-1017 Berlin, Federal Republic of Germany;(3) WB Bioprozeßtechnik, Fakultät für Lebensmitteltechnologie, Humboldt-Universität zu Berlin, Max-Reimann-Strasse 16, O-1532 Kleinmachnow, Federal Republic of Germany;(4) Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18A, W-8033 Martinsried, Federal Republic of Germany |
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| Abstract: | Summary We have analyzed 11 strains and clones, representing five species (Penicillium janthinellum, P. citrioviridae, P. chrysogenum, Aspergillus niger, Trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the DNA of phage M13. The oligonucleotide probes (CT)8, (GTG)5 and (GACA)4, as well as M13 DNA, are informative probes for fingerprinting in all genera and species tested. The probe (GATA)4 produced informative fingerprints only with the genomic DNA of A. niger. There was no similarity between the fingerprints originating from fungi of different genera and also little similarity between the fingerprints of different species belonging to the same genus. Fingerprints of strains of the same species differed only slightly from each other. Fingerprints of clones originating from one strain were identical. The results indicate that DNA fingerprinting is a powerful method to differentiate species and strains of filamentous fungi. |
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| Keywords: | DNA fingerprinting of filamentous fungi Penicillium Trichoderma Aspergillus |
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