| 丹酚酸B对心肌梗死大鼠心肌细胞凋亡的影响 |
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| 引用本文： | 张萌,王怡,王虹,王少侠,柴丽娟. 丹酚酸B对心肌梗死大鼠心肌细胞凋亡的影响[J]. 天津中医药, 2008, 25(4): 318-321 |
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| 作者姓名： | 张萌 王怡 王虹 王少侠 柴丽娟 |
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| 作者单位： | 1. 天津中医药大学中药学院,天津,300193
2. 天津中医药大学中医药研究院,天津,300193 |
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| 基金项目： | 国家重点基础研究发展计划"973"课题资助项目 , 天津市科委自然科学基金项目 |
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| 摘 要： | [目的]研究丹酚酸B(Sal B)对心肌梗死大鼠心肌细胞凋亡的抑制作用及分子机制.[方法]结扎冠脉左前降支复制大鼠心肌梗死模型,假手术组作对照,连续灌胃给药7 d.采用流式细胞术磷脂结合蛋白5(Annexin V)-异硫氰酸荧光素/碘化丙啶(FTTC/PI)法检测心肌细胞凋亡情况,并用实时荧光定量反转录-多聚酶链式反应(RT-PCR)技术检测凋亡相关调控通路的基因表达.[结果]丹酚酸B(Sal B)可以显著降低心肌细胞凋亡率.Sal B可以显著降低Bax、信号转导和转录活化因子(STAT-3)的表达,有降低白细胞介素-6(IL-6)表达的趋势.[结论]Sal B可以有效抑制心肌细胞凋亡,改善心肌梗死后的心脏状况.
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| 关 键 词： | 丹酚酸B 心肌梗死 心肌细胞 凋亡 |
| 收稿时间： | 2008-05-11 |
Effect of salvianolic acid B on apoptosis of myocardial cells in rats with nyocardial infarction |
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| ZHANG Meng,WANG Yi,WANG Hong. Effect of salvianolic acid B on apoptosis of myocardial cells in rats with nyocardial infarction[J]. Tianjin Journal of Traditional Chin Medicine, 2008, 25(4): 318-321 |
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| Authors: | ZHANG Meng WANG Yi WANG Hong |
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| Affiliation: | The TCM College of Tianjin University of TCM, Tianjin 300193, China;The Academy of TCM of Tianjin University of TCM, Tianjin 300193, China;The Academy of TCM of Tianjin University of TCM, Tianjin 300193, China;The Academy of TCM of Tianjin University of TCM, Tianjin 300193, China;The Academy of TCM of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract: | [Objective] To observe the inhibitory effects and mechanism of Salvianolic acid B (Sal B) on apoptosis of myocardial cells in rats with acute myocardial infarction. [Methods] We produced myocardial infarction in anaesthetized rats by occluding the left descending coronary artery. Sham-operated rats served as controls. We assayed the myocardial cell apoptosis and the gene expressions by flow cytometry and real-time RT-PCR. [Results] Sal B can decrease the number of apoptotic myocytes and decrease the expression of IL-6, Bax and STAT-3. [Conclusion] Sal B can limit the myocardial cell apoptosis after infarction and the mechanism may be related to the down regulation of the apoptosis relative genes expression. |
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| Keywords: | salvianolic acid B myocardial infarction myocardial cell apoptosis |
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