不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用

目的  探讨不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用。方法  采用酶联免疫吸附试验（ELISA）法测定54例慢性乙型肝炎患者应用普通干扰素治疗（500万单位，隔日皮下注射1次）前和治疗24周后血清中辅助性T淋巴细胞（Th）17、1、2分泌的主要细胞因子白细胞介素17（IL-17）、γ干扰素（IFN-γ）、白细胞介素4（IL-4）的水平，并根据应答的不同进行分组，分析不同亚型CD4+T淋巴细胞的作用。结果    慢性乙型肝炎患者经普通干扰素治疗24周后IL-17水平下降（P＞0.05），IFN γ水平上升(P＞0.05)，IL 4水平明显下降(P＜0.01)。而在干扰素治疗的完全应答组，IL-17、IL-4水平下降更明显(P＜0.05、P＜0.01 )，IFN γ水平则明显升高(P＜0.01)。结论    慢性乙型肝炎患者经干扰素抗病毒治疗，体内Th17和Th2的功能下调，Th1功能上调。

Roles of different subtypes of CD4+ T lymphocytes in IFN-α therapy for hepatitis B

Objective To study the roles of different subtypes of CD4~+ T lymphocytes in IFN-α therapy for hepatitis B. Methods Th17, Th1 and Th2 cytokines were determined by enzyme-linked immunosorbent assay(ELISA) in 54 hepatitis B patients before and 24 weeks after IFN-a treatment. Patients were grouped according to responses to IFN-α therapy. The data were analyzed by t-test and Pearson correlation analysis. Results The level of IL-17 was slightly lower 24 weeks after IFN-α treatment than that at pre-treatment(P>0.05) ; the level of IFN-γ was slightly increased (P>0.05) ; and the level of IL-4 was substantially decreased(P<0.01). In patients with a complete response to IFN-α, the level of IFN-γ was obviously increased(P<0.01) ; and the levels of IL-17 and IL-4 were significantly decreased (P<0.05; P<0.01). Conclusion During IFN-α therapy, the functions of Th17 and Th2 are down-regulated and the function of Th1 is up-regulated in chronic hepatitis B.