夏枯草总黄酮提取方法及其在居群间分布特征研究

目的:探讨夏枯草总黄酮的最佳提取工艺及其在不同居群、不同部位间分布情况。方法:应用响应面法确定最佳提取工艺,并对不同居群、不同部位中总黄酮含量进行测定。结果:液固比为25∶1,以35%的乙醇在86℃水温下回流提取3.5 h,可获得夏枯草总黄酮的最佳提取效果。不同居群总黄酮含量变异幅度为2.16%~10.29%;叶片中总黄酮含量最高,根中最低;果穗去除种子后总黄酮含量平均高出27.6%。结论:响应面法对夏枯草总黄酮的提取条件优化合理可行;不同居群间总黄酮含量表现出明显的地理分布差异。

Study on flavonoids distribution of in various populations of Prunella vulgaris

OBJECTIVE: To study the extraction flavonoids distribution in different parts of Prunella vulgaris from different populations. METHOD: The optimal extraction condition was selected by response surface method (RSM). The amounts of flavonoids were determined by colorimetric method. RESULT: The optimum extraction condition was using 35% ethanol at 25 times of sawple volume, refluxing at 87 degrees C for 3.5 h. The amount of flavonoids was 2.16% -10.29% in P. vulgaris. The content of flavonoids was the highest in leaf while that in root was the lowest. And the content of flavonoids in spike after removed seeds was 27.6% higher than the one reserved seeds. CONCLUSION: The RSM was feasible for optimum extraction condition and the amount of flavonoids showed a signiticant regional distribution pattern in various populations of P. vulgaris.