微小RNA分子miR-21和miR-22对C2C12细胞成肌分化影响的研究

目的探讨微小RNA（microRNAs）分子miR-21和miR-22对于C2C12细胞体外成肌分化进程的影响。方法以C2C12细胞体外成肌分化为模型，Northern blot检测miR-21和miR-22两个分子在分化过程中的表达变化及组织表达谱，转染DNA／LNA杂合体反义链进行loss-of-fuction功能研究，RT-PCR分析其对分化相关分子的影响。结果成功建立C2C12细胞体外成肌分化模型，2个分子在分化过程中均表达上调，且它们具有各自的组织分布特点；建立了以DNA／LNA杂合体反义链为手段的microRNAs研究的loss-of-fuction平台；与对照组比较，抑制这2个microRNAs对于成肌分化相关的骨骼肌α-肌动蛋白、成肌素和MEF-2D等分子的表达没有影响。结论抑制miR-21和miR-22在C2C12细胞成肌分化过程中的上调不影响其分化，说明这2个分子对于分化的进程影响不大。

Roles of miR-21 and miR-22 in myogenic differentiation of myoblasts C2 C12

Objective To explore the roles of 2 microRNA molecules,miR-21and miR-22,in the myogenic differentiation of C2C12 myoblasts.Methods C2C12 cells was taken as the model since they have a tendency of myogenic differentiation when cultured in differentiation medium.The expression levels of miR-21 and miR-22 in the process of myogenic differentiation and different tissues(the brain,small intestine,skeleton muscle,spleen,kidneys,liver,heart and lungs)were detected by Northern blotting.Loss-of-function about these ...