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双波长薄层扫描法测定金樱子中总黄酮的含量
引用本文:卢静华,于玲. 双波长薄层扫描法测定金樱子中总黄酮的含量[J]. 中国药房, 2008, 19(21): 1636-1637
作者姓名:卢静华  于玲
作者单位:辽宁医学院药学院,锦州市,121001
摘    要:
目的:建立以双波长薄层扫描法测定金樱子中总黄酮含量的方法。方法:以芦丁为标准品,采用双波长飞点薄层扫描仪测定,展开剂为乙酸乙酯-甲酸-水(8:1:1),λs=266nm,λR=230nm。结果:金樱子点样量在1~6μg范围内与峰面积积分值呈良好线性关系;平均回收率为98.9%,RSD=1.39%(n=6)。结论:本方法操作简便、结果准确,可用于金樱子的质量控制。

关 键 词:双波长薄层扫描法  金樱子  总黄酮  含量测定

Determination of Total Flavonoid in Fructus Rosae Laevigatae by Dual Wavelength TLC - Scanning
LU Jing-hua,YU Ling. Determination of Total Flavonoid in Fructus Rosae Laevigatae by Dual Wavelength TLC - Scanning[J]. China Pharmacy, 2008, 19(21): 1636-1637
Authors:LU Jing-hua  YU Ling
Affiliation:(College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China)
Abstract:
OBJECTIVE: To determine the content of the total flavonoid in Fructus Rosae Laevigatae. METHODS: Dual wavelength TLC scanner was selected with rutin as standard substance. The developing solvent was acetic ether- formic acid - water(8 : 1 : 1) ;λ2= 266 nm, and λR = 230 nm. RESULTS: The linear range of Fructus Rosae Laevigatae was 1--6 μg, and the average recovery was 98,9% 2. 397% (RSD = 1,39%, n = 6). CONCLUSION: The method is simple and accurate, and it is applicable for the quality control of Fructus Rosae Laevigatae,
Keywords:Dual wavelength TLC-scanning  Fructus Rosae Laevigatae  Total flavonoid  Content determination
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