氰戊菊酯对小鼠精子顶体反应影响及作用机理

目的　观察氰戊菊酯对小鼠精子顶体反应的影响并探讨其作用机理。方法　采用顶体反应诱导试验结合细胞染毒 ,以考马斯亮蓝染色法判定精子顶体反应。结果　孕酮或A2 3187诱导的小鼠精子顶体反应 ,可被 2mmol·L- 1EDTA和 1.8mmol·L- 1Mn2 +相应地完全抑制 ;5 0 μmol·L- 1氰戊菊酯则有明显抑制作用。实验还发现氰戊菊酯在精子获能前或后加入 ,对孕酮诱发顶体反应的抑制率不同 ,获能前抑制率低 ,二者存在显著性差异。结论　氰戊菊酯对小鼠精子顶体反应有抑制作用 ,对小鼠精子获能可能有一定的促进作用

Effect of fenvalerate on sperm acrosome reaction in mice and its mechanism

AIM To observe the effect of fenvalerate, a pyrethroid insecticide on sperm acrosome reaction in mice, and to study the mechanism of its action. METHODS The sperms treated directly with fenvalerate were stimulated by progesterone or Ca²⁺ ionophore A23187, an inducer of acrosome reaction(AR). The acrosomal status was determined by light microscopy combined with Coomassie brilliant blue staining. The rate of AR of sperm was calculated. RESULTS The rise of intracellular Ca²⁺ was essential in the sperm AR induced by progesterone or A23187, but the rate of AR was obviously lower when the system existed 50 μmol·L^-1 fenvalerate (P<0.01). Furthermore, the study showed that the inhibition rate of AR as adding fenvalerate before capacitation was significantly lower than that after capacitation (P<0.01). CONCLUSION Fenvalerate inhibits sperm AR and may promote sperm capacitation.