高压氧对大鼠局灶性脑缺血/再灌注脑线粒体能量代谢的影响

目的:探讨高压氧(HBO)对大鼠局灶性脑缺血/再灌注(IR)后脑线粒体三磷酸腺苷(ATP)生成量及质子转移性三磷酸腺苷合成酶(F0F1-ATPase)活性的影响.方法:用线栓法阻断大脑中动脉(MCA)的大鼠局灶性IR模型.用Clark的Ficoll密度梯度法分离纯化的线粒体,用生化方法测定线粒体F0F1-ATPase活性,用高效液相色谱技术(HPLC)测定脑线粒体腺苷酸含量.Wistar大鼠30只,随机分为3组:正常对照组(normal)6只,缺血/再灌注组(IR组)12只,高压氧治疗组12只.IR组、HBO组缺血时间均为2h,两组均等分为再灌注2h、24h(I2hR2h、I2hR24h、HBO-I2hR2h、HBO-I2hR24h2组;IR组用1个大气压(ATA)O2治疗;HBO组用2.5ATAO2治疗.结果:HBO治疗后ATP生成量及F0F1-ATPase活性增加;IR组ATP生成量、F0F1-ATPase活性明显低于HBO组.结论:HBO可能是通过恢复F0F1-ATPase的活性,增加ATP合成而改善脑线粒体有氧代谢,减轻缺血性脑损害,保护脑组织的.

Effects of hyperbaric oxygen on cerebral mitochondrial energy metabolism after brain ischemia/reperfusion in rats

Objective:To study the effects of hyperbaric oxygen(HBO) on the levels of cerebral mitochondrial adenosine triphosphate (ATP) and proton-translocating ATP synthases(F 0F 1-ATPase) after brain ischemia/reperfusion (IR) in rats.Methods:Thirty Wistar rats were randomly divided into 3 groups:normal contorl group (n=6) ,IR group (n=12) and HBO group ( n=12 ).IR was performed by ligation of the middle cerebral artery for 2 hours (hs) and then reperfusion in both the IR and HBO groups.The IR group was subdivided into group I 2h R 2h and group I 2h R 24h .The HBO group was subdivided into group HBO-I 2h R 2h and group HBO-I 2h R 24h .The IR group was treated by 2.5 ATAO 2.The HBO group was treated by 2.5 ATA O 2.The pure mitochondria was separated by Clak's Ficoll density gradient method.The activity of F 0F 1-ATPase was determined by biochemical technigue and the level of cerebral ATP by high-performance liquid chromatography (HPLC).Results:The level of ATP and the activity of F 0F 1-ATPase increased after HBO treatment.In the IR group,the level of ATP and the activity of F 0F 1-ATPase were significantly lower than those of the HBO group( P <0.01).Conclusion:HBO improves mitochondrial oxygen metabolism,alleviates cerebral ischemic injury and protects brain tissues by means of recovering the activity of F 0F 1-ATPase and increasing the synthesis of ATP.