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Spontaneous haploidization in early zygote progeny and its use for mapping in the yeast Yarrowia lipolytica
Authors:Cornelia Kurischko
Affiliation:(1) Forschungsbereich Biowissenschaften und Medizin, Zentralinstitut für Mikrobiologie und experimentelle Therapie, Akademie der Wissenschaften der DDR, Beutenbergstrasse 11, DDR-69 Jena
Abstract:
Summary By means of spontaneous haploidization immediately after conjugation, it is possible to map genes in Y. lipolytica. The already known linkages argA — leuA and metA — lysA were confirmed by means of this method. The mating type locus (MAT) is located on the same chromosome as argA — leuA.(Abbreviations: A and B, alleles of the mating type locus; degR, recessive resistance to 2-deoxygiucose (DEG) when acetate is given as carbon source.)
Keywords:Yeast Yarrowia lipolytica  Spontaneous haploidization  Mapping
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