人rIL-2对鼠脾细胞杀瘤功能的影响

目的探讨ｒＩＬ－２抗肿瘤机制。方法用“ＬＤＨ释放法”检测经不同处理的脾细胞对其敏感的靶细胞ＹＡＣ－１，不敏感的ｌｅｗｉｓ肺癌（ＬＬＣ）及耐受的ＬＬＣ肺转移细胞（ＬＬＣＦ１）体外杀伤功能。结果正常脾细胞对３种靶细胞的杀伤能力有明显差异（Ｐ＜０．０１）；经人ｒＩＬ－２体外激活后（ＬＡＫ细胞），杀瘤能力明显增强，并对ＬＬＣ、ＬＬＣＦ１细胞的杀伤作用已无明显差异；对ＬＬＣ血道转移也有明显抑制作用；激活时间对脾细胞杀瘤能力也有影响。结论人ｒＩＬ－２能激活小鼠脾细胞，使其杀瘤尤其是对具有抗ＮＫ细胞的肿瘤杀伤能力明显增强，对肿瘤转移也有疗效。

Effect of human rIL 2 on tumor cytotoxicity murine splenic cells DENG Song Hua,WANG Si Ying,CHENG Jie,ZHANG Bao Ting,

AIM To study the antitumor mechnism of human rIL 2. METHODS The cytotoxicity against YAC 1, LLC, LLCF1 cell of murine splenic cell treated differently is assayed by “lactic acid dehydrogense release” method. RESULTS The cytotoxicities of normal murine splenic cells against three target cells are significantly variant (P<001). After being activated by human rIL 2 in vitro (as LAK cell), its antitumor activity increased, especially to LLCF1 cells. Being activated in different time, the cytolysis of LAK cells against targets is various. LAK cells can inhibit the LLC murine experimental pulmonary metastasis in vivo. CONCLUSION These results indicated the LLC murine splenic cells can be activated by human rIL 2, and antitumor activity increases, especially to the tumor cell against NK cells. LAK cells are found to be effective in the therapy of LLC lung metastases.