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| 肾上腺素诱导兔血小板聚集的实践与理论探讨 | |
| 引用本文： | 储钟禄 张桐芳. 肾上腺素诱导兔血小板聚集的实践与理论探讨[J]. 中国药理学通报, 1998, 14(6): 509-512 |
| 作者姓名： | 储钟禄 张桐芳 |
| 作者单位： | 第二军医大学生化教研室，第二军医大学附属长征医院血库 |
| 摘 要： | 目的创建以Ａｄｒ诱导兔血小板聚集的方法，并对受体分子特性作初步探讨。方法以高Ｋ＋缓冲液等取代兔ＰＲＰ中血浆，以Ａｄｒ诱导聚集，以Ａｐｙｒ证实结果。结果兔血小板悬浮于高Ｋ＋缓冲液时Ａｄｒ能单独诱导真正的聚集。结论由此推测血小板膜上α２肾上腺素受体分子可能为由两种亚单位组成：促聚亚单位和辅助亚单位。人辅助亚单位当Ａｄｒ浓度高达聚集阈值以上时可被激活，与促聚亚单位结合为活性的二聚体，与Ａｄｒ进一步结合发生聚集作用。兔辅助亚单位则还需要高Ｋ＋方能被激活 |
| 关 键 词： | 兔血小板聚集作用；肾上腺素；α_2肾上腺素受体；高K~＋缓冲液；促聚亚单位；辅助亚单位 |
The practice and theoretical exploration on rabbit platelet aggregation induced by adrenaline |
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| CHU Zhong Lu,SONG Chun Qiao,ZHANG Tong Fang ,CHEN Yu Gui ,HUANG Cai Guo. The practice and theoretical exploration on rabbit platelet aggregation induced by adrenaline[J]. Chinese Pharmacological Bulletin, 1998, 14(6): 509-512 | |
| Authors: | CHU Zhong Lu SONG Chun Qiao ZHANG Tong Fang CHEN Yu Gui HUANG Cai Guo |
| Affiliation: | CHU Zhong Lu,SONG Chun Qiao,ZHANG Tong Fang 1,CHEN Yu Gui 2,HUANG Cai Guo 3 |
| Abstract: | AIM To create a method to induce rabbit platelet aggregation by adrenaline , and to explore theoretically the characteristics of the receptor molecule . METHOD To replace the blood[KG*4/5] plasma in rabbit PRP with high K + buffer , etc . for inducing platelet aggregation by adrenaline and adding apyrase to verify the results. RESULTS When rabbit platelets were suspended in high K + buffer the platelet aggregation was really induced by adrenaline alone. CONCLUSION It is supposed that α 2 adrenoceptor molecule on platelet membrane might contain two subunits |
| Keywords: | rabbit platelet aggregation adrenaline α 2 adrenoceptor high K + buffer proaggregatory subunit auxiliary subunit |
| 本文献已被 CNKI 维普 等数据库收录！ | |