非霍奇金淋巴瘤患者CD44,PCNA,vWF：Ag表达及其与恶性度的关系

目的:探讨CD_(44)、增殖细胞核抗原(PCNA)、vWF:Ag在正常淋巴组织及不同恶性度非霍奇金淋巴瘤(NHL)中的表达规律及意义。方法:采用免疫组织化学LSAB法。结果:①在正常淋巴组织中,分化趋于成熟的小淋巴细胞CD_(44)表达呈强阳性,而生发中心处于转化阶段的淋巴细胞CD_(44)不表达。②不同恶性度NHL均不同水平表达CD_(44),其中高度及中度恶性者CD_(44)呈强阳性,高度恶性者阳性率高于中度恶性者,其差异具有显著性(P<0.05);10例低度恶性者中,只有2例弱表达,其余均为阴性,与前二者之间的差异均具有显著性(P<0.01)。PCNA的表达也呈现上述特点。③不同恶性度NHL的微血管密度随其恶性度的增高而逐渐升高,各级别之间的差异均具有显著性(P<0.05)。结论:CD_(44)在正常淋巴组织及NHL组织中具有不同的表达规律;把NHL中CD_(44)的表达与PCNA的表达及血管密度相结合,可作为判定NHL恶性度的可靠指标。

The expression of CD_(44), PCNA, vWF: Ag in non-Hodgkin''s lymphoma and its relationship with the grade of malignancy

OBJECTIVE: To study the expression of CD44, proliferating cell nuclear antigen (PCNA), vWF: Ag in normal lymphatic tissue and non-Hodgkin's lymphoma (NHL) and its implication. METHODS: Immunohistochemical assay was performed with a LSAB kit. RESULTS: 1. In normal lymphatic tissues, mature appearing small lymphocytes were positive for CD44, while proliferating immature lymphocytes in germinal center were negative. 2. High grade and intermediate grade NHL samples were strongly positive for CD44, the positive rate in the former was higher than in the latter (P < 0.05). In the ten low grade lymphoma samples, only two were weakly positive and, the rest were negative. The expression of PCNA showed the same manner as CD44. 3. The microvessels in NHL increased with the histological malignant grade. CONCLUSION: The expression of CD44 in normal lymphatic tissues is different from that in NHL. Combination of CD44 expression with PCNA expression and microvessel density seems a reliable parameter for judging malignant degree of NHL.