多发性骨髓瘤患者骨髓细胞IL-6分泌机制的研究

目的：探讨多发性骨髓瘤（ＭＭ）患者骨髓细胞中白细胞介素６（ＩＬ－６）分泌机制。方法：应用双标记免疫荧光技术检测了１６例ＭＭ患者和１９例对照者新鲜分离的骨髓单个核细胞中ＩＬ－６表达情况。结果：ＭＭ患者骨髓单个核细胞ＩＬ－６表达阳性率为１５．４％±６．５％，骨髓瘤细胞ＩＬ－６表达阳性率达７．９％±２．９％，基质细胞ＩＬ－６表达阳性率达４．５％±１．２％；对照组骨髓单个核细胞ＩＬ－６表达阳性率为４．６％±２．３％，基质细胞ＩＬ－６表达阳性率为２．６％±１．１％，浆细胞ＩＬ－６表达阳性率为０．７％±０．１％。ＭＭ患者均较对照组显著升高（Ｐ均＜０．００１）。另外ＭＭ组骨髓单个核细胞中基质细胞所占比率为６．９％±１．４％，也较对照组的４．７％±１．８％明显升高（Ｐ＜０．００１）。结论：ＭＭ患者升高的ＩＬ－６既可由瘤细胞分泌，又可由基质细胞分泌，自分泌和旁分泌机制可能都参与了ＭＭ的发病。

Mechanism of IL-6 secretion in bone marrow cells from multiple myeloma patients

OBJECTIVE: To explore the mechanism of interleukin-6(IL-6) secretion in bone marrow cells from patients with multiple myeloma(MM). METHODS: Expression of IL-6 in bone marrow mononuclear cells(BMMNC) from MM patients(n = 16) and controls(n = 19) was detected by using double labelled immunofluorescence. RESULTS: In MM group, the positive rates of IL-6 expression were 15.4% +/- 6.5% in BMMNC, 7.9% +/- 2.9% in myeloma cells, and 4.5% +/- 1.2% in stromal cells, while in controls the positive rates were 4.6% +/- 2.3% in BMMNC, 2.6% +/- 1.1% in stromal cells and 0.7% +/- 0.1% in plasma cells, respectively. The positive rates in MM group were all significantly higher than those in controls(P < 0.001). The cytoplasmic IgG+ and vWF+ cells percentages in BMMNC were also significantly higher in MM group than those in controls. CONCLUSION: Elevated IL-6 in MM patients was secreted by both myeloma cells and stromal cells, and the mechanism of both autocrine and paracrine may be involved in pathogeneses of multiple myeloma.