胰腺癌组织中COX-2的表达与其生物学行为关系的研究

目的通过检测环氧合酶2(COX2)在不同胰腺组织中的表达,探讨COX2在胰腺癌发生发展过程中的作用及与其生物学行为和预后的关系。方法应用免疫组织化学方法检测47例胰腺癌,12例胰腺导管上皮内肿瘤(PIN)和10例正常胰腺组织中COX2的表达。结果在10例正常胰腺组织中COX2阳性表达2例;COX2在PIN和胰腺癌中均呈高表达,分别为75%和68%,明显高于正常胰腺组织(P<0.05)。COX2在高分化、中分化和低分化胰腺癌组织中阳性表达率依次增高,分别为40%、69%和81%,但仅高分化与低分化组间差异有显著性(P<0.05);有淋巴结转移组COX2的阳性率为84%,明显高于无淋巴结转移组的50%(P<0.05)。COX2的表达与胰腺癌的部位、大小、是否浸润周围组织和是否发生远处转移无关(P>0.05),但与病人术后的生存期有关。生存超过1年组COX阳性表达率为38%,明显低于生存期不足1年组的79%,差异有显著性(P<0.05)。结论COX2在PIN和胰腺癌组织中高表达与胰腺癌的发生密切相关,对预测胰腺癌的分化程度、是否易发生淋巴结转移及病人的预后均有一定的作用,在临床上是具有较好指导意义的分子生物学指标。

The Relation Between the Expression of Cyciooxygenase-2 and Biologic Behavior of Human Pancreatic Cancer

Objectives To investigate the expression of cyclooxygenase-2(COX-2) in different pancreatic tissues in order to explore its role and significance in development of pancreatic cancer. Methods Immunohistochelistry staining (Picture TM two steps method) was used to detect expression of COX-2 in 47 cases of paraffin-embedded pancreatic carcinomas tissue, 12 of pancreatic intraductal neoplasias(PIN) and 10 of normal pancreatic tissues. Results The positive expression of COX-2 was noted in 2 out of 10 cases of normal pancreatic tissues; the high expressions of COX-2 were found in PIN and pancreatic carcinomas(75%,68% respectively), which were significantly higher than that in normal pancreatic tissues respectively (P<0.05). The rastes of COX-2 expression in well-differentiated, moderately-differentiated and poorly-differentiated pancreatic carcinoma tissues were 40%,69% and 81%. The significant difference was observed between well-differentiated and poorly-differentiated pancreatic carcinomas tissues (P<0.05). The expression of COX-2 was as high as 84% in pancreatic carcinomas with lymph node metastasis, which was significantly higher than those without lymph node metastasis (P<0.05). Otherwise, no significant correlation was noted between COX-2 expression and the location, size, adjacent tissues invasion, and distant metastasis of pancreatic carcinomas(P>0.05). The positive expression of COX-2 was 38% among those patients suffering from pancreatic carcinoma with 1-year post-operation survival, which was lower than those with less than 1-year post-operation survival(P<0.05). Conclusion COX-2 expression in PIN and pancreatic carcinomas may be involved in the carcinogenesis of pancreatic cancer. COX-2 may be an useful index to predict the differentiated level, lymphoid metastasis and prognosis of pancreatic cancer.