| 人与大鼠补体C5b—9复合物的提取及相应抗体的制备 |
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| 引用本文： | 王迎伟,赵文君.人与大鼠补体C5b—9复合物的提取及相应抗体的制备[J].徐州医学院学报,1999,19(5):353-355. |
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| 作者姓名： | 王迎伟 赵文君 |
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| 作者单位： | [1]徐州医学院免疫学教研室 [2]徐州医学院生物化学教研室 |
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| 基金项目： | 江苏省及省教委自然科学基金!BK9715 4,No .96 0 95 |
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| 摘 要： | 目的 提取人及大鼠血清补体Ｃ５ｂ－ｐ复合物并制备抗体。方法 利用兔红细胞能直接活化人补体旁路途径的特性首先获得人或大鼠补体攻击破裂的兔红细胞膜，然后用脱氧胆酸钠溶解后的经超速离心提取Ｃ５ｂ－９复合物。结果 纯化的Ｃ５ｂ－９复合物电镜负染观察有其独特的形态。
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| 关 键 词： | 补体 C5b－9复合物 提取 鉴定 |
C_(5b-9) COMPLEX OF HUMAN OR RAT COMPLEMENT: ISOLATION FROM COMPLEMENT LYSED CELLS AND PREPARATION OF CORRESPONDING ANTIBODY |
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| Wang Yingwei,Tang Renxian,Zhao Wenjun.C_(5b-9) COMPLEX OF HUMAN OR RAT COMPLEMENT: ISOLATION FROM COMPLEMENT LYSED CELLS AND PREPARATION OF CORRESPONDING ANTIBODY[J].Acta Academiae Medicinae Xuzhou,1999,19(5):353-355. |
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| Authors: | Wang Yingwei Tang Renxian Zhao Wenjun |
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| Abstract: | Objective To isolate and identify C 5b-9 complexes from human or rat serum and prepare the corresponding antibody. Methods C 5b-9 complexes were isolated by gradient ultracentrifugation from lysed rabbit RBC membrane which was attacked by complement through alternative pathway.?Results The purified C 5b-9 complexes showed their special conformation under electron microscope and seven bands in SDS-PAGE. The staining of isolated C 5b-9 complexes with anti-C 5 or C 9 or C 5b-9 antibody separately showed positive reaction. Moreover, the specific antibody against rat C 5b-9 complexes was obtained by immunized rabbit with purified C 5b-9 complexes of rats.?Conclusion The method of isolated serum C 5b-9 complexes through complement alternative pathway is very convenient and reliable. |
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| Keywords: | Complement C 5b-9 complex Isolation Identification |
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