动态优化方法在人参提取工艺优化中的应用

目的:采用动态优化方法优选人参中人参皂苷提取工艺。方法:采用HPLC测定人参中人参皂苷Rg1,人参皂苷Re和人参皂苷Rb1的含量变化,Diamonsil RP-18e色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-水(B)梯度洗脱(0~7 min,16%~18%A;7~27 min,18%~30%A),流速3.0 m L·min-1,柱温35℃,DAD检测波长203 nm,进样量20μL。通过比较动态优化工艺与文献提取工艺所得人参提取物中浸膏得率、指标成分含量和指纹图谱的相似度,评价动态优化方法应用于人参中人参皂苷提取工艺优化的可行性。结果:动态优化提取工艺为8倍量70%乙醇提取3次,提取时间为第1次40 min,第2次20 min,第3次40 min,总提取物的浸膏得率为41.3 mg·g-1。动态优化提取工艺与文献提取工艺的提取物中指标成分含量及浸膏得率的RSD均5%,指纹图谱相似度为100%,表明2种提取方法的提取物基本一致,但是动态优化提取工艺提取时间节约63%,耗电量节约60%。结论:动态优化方法可以用于人参中人参皂苷提取的工业化生产,该工艺能大幅减少提取时间,缩短生产周期,降低能耗,能够产生明显的经济价值,具有很好的应用前景。

Application of Dynamic Optimization Method in Extraction Process Optimization of Ginseng Radix et Rhizoma

Objective: To develope a dynamic optimization technique (DOT) method for the extraction of ginsenosides from ginseng slices. Method: The contents of three major ginsenosides (Rg₁, Re and Rb₁) were determined by HPLC. The extraction yield, contents of ginsenosides and fingerprints similarity were compared between the DOT method and literature extraction process, to evaluate the feasibility of DOT in optimizing the extraction of ginsenosides from ginseng slices. Result: The dynamic optimization process was extracting for thrice with 8 times by 70% ethanol, and extracting time was 40, 20, and 40 minutes in turn with a total extract yield of 41.3 mg·g^-1. The extract between DOT process and the literature extraction process was basically the same, but the DOT extraction method could save 63% of extraction time and 60% of power consumption. Conclusion: DOT extraction method can provide shorter extraction time and lower energy cost in the industrial production of ginsenosides from Ginsen Radix et Rhizoma, and it can produce obvious economic value.