Wistar系大鼠群暴发性痘病的研究

在一群Wistar大鼠中发现一起类似小鼠脱脚病的疾病暴发.发病率高达44.5%,病死率为11.3%。在病鼠皮肤、卵巢和子宫细胞中,以及接种病料的鸡胚绒毛尿囊膜(CECM)上皮细胞中检发现大量A型包涵体。病料可诱导大、小鼠患病,可致CECM形成痘斑,使培养的IBRS_2细胞系发生CPE。用含毒IBRS_2细胞培养上清或CECM痘斑组织匀浆回归同系健康大鼠和小鼠,均可复制本病。SPA协同凝集试验证明,所分离病毒的抗原性与致病因子的相同.电镜下病毒粒子形态与痘病毒相似,有囊膜,直径250～350nm。

A Study of Outbreaking Pox Disease in the Colony of Wistar Strain Rats

A ectromelia-like disease in th colony of wistar strain rats was studied. The clinic signs,dermal lesions of the disease rats are similar to mouse pox. The morbidity of this disease is 44.5%. The fatality^ rate is 11.3%. Type A inclusion bodies were observed in dermal dermis and hypodermis tissues of disease rats as well as in the epithelial cells of the chick embryo chori-oallantoic membrane ( C.E. C.M ) with the pox pock induced by inoculation of the tissue suspention from a disease rat. The disease was reproduced artificially in mice and rats which are good health following inoculation with livers,spleens and dermal necrosis tissue suspension of the disease rats.And the virus was isolated and a cytopathogenic effect was observed in IBRS2 cell line. The disease was also reproduced by the culture suspention of IBRS2 cells with the virus or the suspention of homogenized tissue of C.E. C.M which was inoculated with the suspention of the tissues of the disease rats. It is demonstrated by means of the agglutination test coordinated by SPA that the antigenicity of the virue isolated is the same as that of the pathogenic agent. Under the electron microscope viral particles with envelope were morphologically similar to poxvirus and 250-350 nm in diameter.