利用昆虫-杆状病毒表达系统表达人乳头瘤病毒16型L1蛋白

目的 获得人乳头瘤病毒16型（Human papillomavirus type 16,HPV16)L1蛋白。方法 以pFB1为载体构建HPV16L1杆状病毒表达质 ，并感染昆虫细胞Sf9结果 收集27℃，培养72h的感染重组病毒的Sf9，提取细胞蛋白。经SDS－PAGE蛋白电泳分析，发现有一相对分子质量为56000的蛋白表达，Western blot证实所表达的蛋白为HPV16L1。结论 昆虫一杆状病毒表达系统可有效地表达HPV16L1蛋白。

Production of HPV 16 major capsid protein L1 with baculovirus expression system

BACKGROUND: To obtain human papillomavirus type 16(HPV 16) major capsid protein L1 with baculovirus expression system. METHODS: Using pFb1 as a vector,a recombinant baculovirus expressing plasmid,which contains HPV16L1 gene sequence,was generated.The constructed virus was infected into an insect cell line Sf9. RESULTS: After incubating at 27 degrees for 72 hours, the infected cells were collected and total cellular poteins were extracted. SDS-PAGE assay revealed a roughly 56 000 expressed protein and Western blot confirmed that the expressed protein arose from HPV16L1. CONCLUSIONS: HPV16 later protein L1 could be efficiently expressed with baculovirus expression system,and the expressed L1 protein remains to have good immunoreactivity. This study may supply a basic work for preparing virus-like particle and prophylactic HPV subunit vaccines.