CTGF反义寡核苷酸对人肾小管上皮细胞转分化的影响

目的研究结缔组织生长因子（CTGF）反义寡核苷酸（ASODN）对人肾小管上皮细胞（HKC）转分化的影响。方法用巨噬细胞趋化因子-1（MCP-1）和马兜铃酸Ⅰ（AAⅠ）联合诱导HKC转分化模型。实验设空白对照组、模型对照组、PEI组、CTGFASODN10ng/mL组和CTGF ASODN100ng/mL组,分别采用WST-8法、流式细胞术、RT-PCR法、细胞免疫组织化学法检测不同浓度的CTGF ASODN对HKC转分化模型细胞增殖和细胞周期的影响,以及α-SMA mRNA表达和α-SMA、TGF-β1、Fibronectin（FN）蛋白表达的变化。结果 100ng/mL的CTGF ASODN对MCP-1和AAⅠ联合诱导HKC转分化细胞具有促进细胞增殖的作用,并可减少实验模型导致的细胞S期阻滞,降低α-SMA mRNA表达,减少α-SMA、TGF-β1和FN蛋白的表达。结论 CTGFASODN能够抑制MCP-1和AAⅠ导致的人HKC转分化。

Effects of CTGF antisense oligodeoxynucleotide on transition of human kidney epithelial cells

Objective To investigate the effects of antisense oligodeoxynucleotide (ASODN) targeting connective tissue growth factor (CTGF) on transition of human kidney epithelial cells (HKC). Methods Transition models of HKC were induced by monocyte chemotactic protein-1(MCP-1) and aristolochic acid Ⅰ(AAⅠ). HKC were randomly divided into blank control group, model control group, PEI group, CTGF ASODN 10 ng/mL group and CTGF ASODN 100 ng/mL group. The effects of CTGF ASODN on proliferation and cell cycle of transition models of HKC were evaluated by WST-8 method and flow cytometry, respectively. The expression of α-smooth muscle actin (α-SMA) mRNA and α-SMA, Fibronectin (FN) and TGF-β1 protein of transition models of HKC were detected by RT-PCR and immunohistochemistry, respectively. Results 100 ng/mL CTGF ASODN reversed the proliferation inhibition, reduced S phase arrest, decreased the expression of α-SMA mRNA and α-SMA, FN and TGF-β1 protein of HKC induced by MCP-1 and AAⅠ. Conclusion CTGF ASODN can inhibit the transition of HKC induced by MCP-1 and AAⅠ.